| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 372,861 | G→A | 100% | A1270T (GCG→ACG) | entF → | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 372,861 | 0 | G | A | 86.7% | 36.2 / 0.0 | 15 | A1270T (GCG→ACG) | entF | enterobactin synthase multienzyme complex component, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (13/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.52e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCC > minE/372799‑372907 | tGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGTATTGTGCGCAtgtg < 1:754316/71‑1 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGttt > 1:1635541/1‑64 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:136239/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:1371655/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:184096/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:2224021/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:2323033/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:2596300/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:2853935/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:512277/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:513936/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:651916/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:70735/1‑71 (MQ=255) aGCGGAGCTGGATATCTATCGTCAGGATTGTACGCATGTGGATATTATCTCTCCAGGGACGTTTGaaaaaa > 1:924386/1‑71 (MQ=255) cTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGcc < 1:2366309/71‑1 (MQ=255) | TGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCC > minE/372799‑372907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |