| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 484,358 | G→A | 100% | G55D (GGC→GAC) | tolB → | periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 484,358 | 0 | G | A | 90.6% | 92.8 / 2.1 | 32 | G55D (GGC→GAC) | tolB | periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (29/0); total (32/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCCACA > minE/484312‑484427 | gttCCTTTCCAGTGGGCTGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:274294/1‑71 (MQ=255)gttCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:2886427/1‑71 (MQ=255)gttCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCg > 1:855958/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGc > 1:2138843/1‑64 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTaaa > 1:296101/1‑70 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1647418/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2255289/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1694964/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1793604/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:50741/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:187200/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1894970/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2365508/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:404046/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:395395/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:367855/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2142325/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2153626/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2195419/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1616369/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1044793/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1462820/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:609183/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1428636/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1375557/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:244365/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:522404/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1274778/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:893081/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:1190839/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2557745/1‑71 (MQ=255) ggCGGGGCCTGGTGCGGCACCTGAAGATATTGACGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAAt > 1:2695901/1‑71 (MQ=255) ggcggcATGGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2445603/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGcc > 1:2916466/1‑68 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGc > 1:407856/1‑67 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCcacg > 1:2772258/1‑70 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCcac > 1:391074/1‑70 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:412042/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:603397/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:663126/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:680861/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:342665/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:725430/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:847798/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:89564/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:917964/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2798539/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:278859/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:93342/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:968337/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:238167/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1628932/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1134384/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1139744/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1166572/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1229049/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1230393/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:12571/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1261433/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1293609/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1309864/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1368543/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1452069/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1536329/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2611000/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1797397/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:1923668/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2032605/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2075731/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2087631/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2134358/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2312437/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2459043/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:251012/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:251120/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:2543120/1‑69 (MQ=255) ggcggcATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCca > 1:260872/1‑69 (MQ=255) | GTTCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATATTGGCGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCCACA > minE/484312‑484427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |