| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 599,045 | A→G | 100% | D463G (GAT→GGT) | dmsA → | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 599,045 | 0 | A | G | 81.8% | 21.3 / 0.1 | 11 | D463G (GAT→GGT) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (0/9); total (0/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGTGGCCCGGAAATGACGGCGCTGCGTGATGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGA > minE/599015‑599089 | aaCGTGGCCCGGAAATGACGGCGCTGCGTGATGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCaa < 1:2099155/70‑1 (MQ=255)aaCGTGGCCCGGAAATGACGGCGCTGCGTGATGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCaa < 1:2136720/70‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:1173726/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:1273981/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:1286585/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:1314380/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:1987189/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:2403208/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:2502245/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:2538046/71‑1 (MQ=255) tGGCCCGGAAATGACGGCGCTGCGTGGTGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGa < 1:856955/71‑1 (MQ=255) | AACGTGGCCCGGAAATGACGGCGCTGCGTGATGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGA > minE/599015‑599089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |