Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	656,044	T→C	100%	F44L (TTC→CTC)	ycbS →	predicted outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	656,044	0	T	C	79.0%	34.5 / 6.1	19	F44L (TTC→CTC)	ycbS	predicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base C (0/15);  total (4/15)
Fisher's exact test for biased strand distribution p-value = 2.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACA  >  minE/655990‑656096                                                       |                                                     tttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAga                                               >  1:183472/1‑62 (MQ=255) tttGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATACCt                                       >  1:2252913/1‑70 (MQ=255)   tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCaa                                    >  1:1453427/1‑71 (MQ=255)   tgtgTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCaa                                    >  1:2180887/1‑71 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2145208/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:494339/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:381850/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2405010/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2382286/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2379433/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2276746/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:2227129/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:102089/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1937403/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1790798/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1629366/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1605367/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1238338/69‑1 (MQ=255)                                       gTTCGATCCTGCTTTTCTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACa  <  1:1155392/69‑1 (MQ=255)                                                       |                                                     TTTGTGTCGGCAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCCGATTTGTCTCGTTTCGAAAAAGGACA  >  minE/655990‑656096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑