| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,172 | A→G | 43.6% | N665N (AAT→AAC) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,172 | 0 | A | G | 43.6% | 79.0 / 233.9 | 172 | N665N (AAT→AAC) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (56/41); new base G (67/8); total (123/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 173 total aligned reads displayed.
CAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAT > minE/138106‑138224 | cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:2206783/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:1568992/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:2290667/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:2374680/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:1133973/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:2779105/1‑71 (MQ=255)cagtcCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTg > 1:28789/1‑71 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:1003351/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:1551453/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:1543943/70‑1 (MQ=255) cACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGa < 1:2590104/70‑1 (MQ=255) ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGGTAt > 1:2030858/1‑61 (MQ=255) ccGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAAt < 1:901224/71‑1 (MQ=255) cGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAAt < 1:447126/70‑1 (MQ=255) cTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:2341473/71‑1 (MQ=255) tATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGa < 1:2260273/70‑1 (MQ=255) aCAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCa > 1:775973/1‑70 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:856017/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1977668/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2051792/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2178894/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1055168/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:222403/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:936014/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:892599/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2355095/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2473130/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2527564/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:684222/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:667162/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:2837481/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:583445/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:353873/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1627541/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1376026/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1465011/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1471466/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1262145/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1545729/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:167672/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1189599/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:107293/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1084345/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1105746/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGaa > 1:2896679/1‑56 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGaa > 1:944650/1‑62 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGcc > 1:207125/1‑68 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:959964/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2641511/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2700573/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2701597/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1330949/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2860918/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:449742/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:482651/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1870597/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:540301/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:845038/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1128169/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1174955/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:765346/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2079589/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2258532/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1779521/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1615602/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2059062/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2338475/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2009778/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:148548/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1810226/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:24330/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1406149/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:1867078/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:2532795/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTCGCAGAAATTGAACCTGCCAGc > 1:1082927/1‑71 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCCCTGTTTGCAGAAATTGAACCTGCCAg > 1:2607216/1‑70 (MQ=255) cAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTAATCGCTGTTTGCAGAAATTGAACCTGCCAGc > 1:1228971/1‑71 (MQ=255) cAAAACAACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGc > 1:3760/1‑71 (MQ=255) aaaCCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGt > 1:2144523/1‑71 (MQ=255) caccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTccc < 1:150523/71‑1 (MQ=255) caccGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTccc < 1:695308/71‑1 (MQ=255) aaCTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTccc < 1:2553373/52‑1 (MQ=255) cTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCa > 1:342905/1‑71 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTTAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1134972/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGGTGGCAt < 1:5274/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1706621/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2016212/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:971081/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:763186/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:753417/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:709993/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2124576/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1659552/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:463708/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1209741/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2919417/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:1232932/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2410488/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2660634/69‑1 (MQ=255) gACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAt < 1:2539557/69‑1 (MQ=255) | CAGTCCACCGCTATGCAATACAAAACCACCGTCGGTGCTGAGAGAAACTTGACGGCTGTTATCGCTATTTGCAGAAATTGAACCTGCCAGCGTTCCCCATGGTGACTCATAGCTGGCAT > minE/138106‑138224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |