| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 710,513 | C→A | 100% | C301F (TGC→TTC) | solA ← | N‑methyltryptophan oxidase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 710,513 | 0 | C | A | 91.3% | 123.5 / 7.6 | 46 | C301F (TGC→TTC) | solA | N‑methyltryptophan oxidase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base A (0/42); total (0/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCATAGGTGCAGGCAGCGCCGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGCC > minE/710482‑710572 | aTCCTAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1199364/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAATAACGGGAAg < 1:1181038/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:368628/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2044047/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2265243/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2458150/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:254457/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2699229/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2709827/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2726037/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2756576/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2856523/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:287108/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2119182/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:40672/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:484292/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:554822/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:60805/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:672305/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:868177/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:916160/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2024708/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1131603/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1984821/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1815991/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1146256/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1224401/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1331711/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1776152/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:144945/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1490563/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1504417/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAAAGGGAAg < 1:4544/70‑1 (MQ=255)aTCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACACGGCAATACATTGCGCAAGAACGGGAAg < 1:206380/70‑1 (MQ=255) tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1589830/69‑1 (MQ=255) tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1665571/69‑1 (MQ=255) tCATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2471052/69‑1 (MQ=255) cATAGGTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1723874/68‑1 (MQ=255) tgTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:2661324/63‑1 (MQ=255) gTGCAGGCAGCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1714729/63‑1 (MQ=255) aggcagCGCCGTACAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:1779520/59‑1 (MQ=255) cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1660417/71‑1 (MQ=255) cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1697483/71‑1 (MQ=255) cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:908786/71‑1 (MQ=255) cGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGcc < 1:1075646/71‑1 (MQ=255) aCAGGAAGAAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAg < 1:633324/47‑1 (MQ=255) | ATCATAGGTGCAGGCAGCGCCGTACAGGCAGCAACCGATACCCGGCAATACATTGCGCAAGAACGGGAAGGCTTCTGACCCATCACTGGCC > minE/710482‑710572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |