| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 813,089 | T→A | 100% | intergenic (‑72/+53) | ychM ← / ← prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 813,089 | 0 | T | A | 100.0% | 21.3 / NA | 8 | intergenic (‑72/+53) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/0); total (8/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCC > minE/813044‑813115 | atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGa > 1:1669998/1‑58 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:1405634/1‑71 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2477258/1‑71 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2582457/1‑71 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:2670660/1‑71 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgc > 1:913709/1‑71 (MQ=255)atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTAAAGACAAAAAAACccgc > 1:846911/1‑71 (MQ=255)atcTGT‑AACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACccgcc > 1:1858073/1‑71 (MQ=255) | ATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCC > minE/813044‑813115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |