Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	813,910	A→G	100%	C60C (TGT→TGC)	prsA ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	813,910	0	A	G	100.0%	111.8 / NA	31	C60C (TGT→TGC)	prsA	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/0);  total (31/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTG  >  minE/813894‑813963                 |                                                      tGTCGTTAGTAGGGGTGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1270909/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGAGTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1460426/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATcacaac                        >  1:77359/1‑48 (MQ=39) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTaa      >  1:949052/1‑66 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAAttt   >  1:2799938/1‑69 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:240175/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2069035/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2459244/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2581116/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2772381/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:496973/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:614475/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:616052/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:669318/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:681686/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:701377/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:941349/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2305866/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1070392/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2057819/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2049762/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2038999/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:2032378/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1799142/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1614039/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1497858/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1485/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:135558/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:12914/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1271376/1‑70 (MQ=255) tGTCGTTAGTAGGGGCGCAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTg  >  1:1077529/1‑70 (MQ=255)                 |                                                      TGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTG  >  minE/813894‑813963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑