| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 893,219 | C→T | 100% | R85H (CGC→CAC) | sapC ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 893,219 | 0 | C | T | 100.0% | 10.1 / NA | 5 | R85H (CGC→CAC) | sapC | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGT > minE/893182‑893228 | tGGCGCAGCTCCGCTCAGTAAACGTCTTAAAACATCGTGTCCGAGgt > 1:1755726/1‑47 (MQ=255)tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGTGTcc > 1:519101/1‑42 (MQ=255)tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGTGTCCGAGgt > 1:1253999/1‑47 (MQ=255)tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGTGTCCGAGgt > 1:2621137/1‑47 (MQ=255)tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGTGTCCGAGgt > 1:2820299/1‑47 (MQ=255) | TGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGT > minE/893182‑893228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |