Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 157,851 A→T 100% V43D (GTT→GAT)  pfs ← 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE157,8510AT100.0% 92.5 / NA 29V43D (GTT→GAT) pfs5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/29);  total (0/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAT  >  minE/157818‑157921
                                 |                                                                      
gCGACTTTACCGATGCTCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2538464/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2251454/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:990871/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:903603/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:896286/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:748132/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:595155/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:573841/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:54058/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2680790/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:261861/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2557835/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1130959/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2196908/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1797830/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1434668/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1447714/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1598139/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1689671/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:170131/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2081935/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1806730/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1839965/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:185464/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1885838/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2034844/71‑1 (MQ=255)
gCGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:2068987/71‑1 (MQ=255)
 cGACTTTACCGATGCCCGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:723315/70‑1 (MQ=255)
              tcccGATTTCAGAAGCGCATCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCa                                   <  1:1573501/56‑1 (MQ=255)
                                 aCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt  <  1:272309/71‑1 (MQ=255)
                                 aCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt  <  1:2329492/71‑1 (MQ=255)
                                 aCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt  <  1:749558/71‑1 (MQ=255)
                                 aCCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt  <  1:752971/71‑1 (MQ=255)
                                 |                                                                      
GCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAT  >  minE/157818‑157921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑