| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,132,803 | G→A | 100% | intergenic (+119/‑38) | arpB → / → yniD | ECK1718:JW5278+JW1710:b4494; hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,132,803 | 0 | G | A | 100.0% | 88.1 / NA | 27 | intergenic (+119/‑38) | arpB/yniD | ECK1718:JW5278+JW1710:b4494; hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/27); total (0/27) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGGCTGTAATAACAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTGG > minE/1132769‑1132854 | gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:2016703/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:954902/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:923434/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:619506/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:586327/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:489387/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:2865168/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:2485260/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:2296020/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:2105213/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1109631/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1953719/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1888647/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1845244/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:155499/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1541895/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1498157/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1428859/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1340414/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1224758/71‑1 (MQ=255)gACGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:118010/71‑1 (MQ=255) aCGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAgaga < 1:1803990/70‑1 (MQ=255) aCGGCTGTAATAACAAAAAATCCCTTGATGCCTATCCCTTTTGATACACTCCGTTATCACGCACAAgaga < 1:1668300/70‑1 (MQ=255) aaaaaaTCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTgg < 1:2428343/71‑1 (MQ=255) aaaaaaTCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTgg < 1:438395/71‑1 (MQ=255) aaaaaaTCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTgg < 1:573651/71‑1 (MQ=255) aaaaaaTCCCTTGATGCCTATCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTgg < 1:620895/71‑1 (MQ=255) | GACGGCTGTAATAACAAAAAATCCCTTGATGCCTGTCCCTTTTGTTACACTCCGTTATCACGCACAAGAGATATGCAGGACACTGG > minE/1132769‑1132854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |