Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,133,843 | T→C | 100% | intergenic (‑69/‑218) | ydiY ← / → pfkB | conserved hypothetical protein/6‑phosphofructokinase II |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,133,843 | 0 | T | C | 81.3% | 111.8 / 20.0 | 48 | intergenic (‑69/‑218) | ydiY/pfkB | conserved hypothetical protein/6‑phosphofructokinase II |
Reads supporting (aligned to +/- strand): ref base T (3/6); new base C (39/0); total (42/6) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.85e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACG > minE/1133776‑1133865 | tATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTa > 1:1673889/1‑70 (MQ=255) tATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAg > 1:2638716/1‑71 (MQ=255) tATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGCATCAAAGATTAg > 1:829772/1‑71 (MQ=255) cGTACAACAAAAAAAGAGCCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2116199/1‑70 (MQ=255) cGTACAACAAAAAAAGAGCCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2523598/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCGTCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:988553/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTgg > 1:2109336/1‑69 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2004787/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2066493/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1008890/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2182409/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:24235/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2616853/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2635436/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2763685/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:277056/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2864112/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:2912400/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:459102/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:665172/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:672258/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:674556/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:741229/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1901593/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1024971/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:103521/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1256106/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1335764/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1403310/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1441305/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1459692/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1512759/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:155990/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1623422/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1633858/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1715780/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1716908/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1719006/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1863374/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1866577/1‑70 (MQ=255) cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:1906818/1‑70 (MQ=255) cGTAAAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGACTAGCGTCCCTGGa > 1:124044/1‑70 (MQ=255) aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg < 1:1451465/71‑1 (MQ=255) aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg < 1:1416512/71‑1 (MQ=255) aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg < 1:21108/71‑1 (MQ=255) aaaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCATGGAAAGGTAACg < 1:2414148/71‑1 (MQ=255) aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg < 1:2719319/70‑1 (MQ=255) aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACg < 1:2738338/70‑1 (MQ=255) | TATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACG > minE/1133776‑1133865 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |