| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,249,224 | C→T | 100% | D17N (GAT→AAT) | ruvB ← | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,249,224 | 0 | C | T | 100.0% | 101.7 / NA | 31 | D17N (GAT→AAT) | ruvB | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (6/25); total (6/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAT > minE/1249168‑1249251 | cGGCTTACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2071205/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCTAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:173519/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTTCTACATTTTCCGGCAAAGt < 1:2568987/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCATAGt < 1:2471868/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2174946/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:962753/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:680663/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:677577/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:564733/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2702697/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2537358/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2465733/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2441667/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2215630/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2172449/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:21181/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2097136/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1025424/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:2086927/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1078684/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:114843/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1159798/69‑1 (MQ=255)cGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGt < 1:1429176/69‑1 (MQ=255) ggCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCTATCTGCTACATTTTCCGGCAAAGt < 1:460983/68‑1 (MQ=255) aCCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGTCAAAGt < 1:1020829/63‑1 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:390300/1‑71 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:17568/1‑71 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:625010/1‑71 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:2549999/1‑71 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:1851306/1‑71 (MQ=255) tACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATTTTCCGGCAAAGTGGTACCGGCAGAAAt > 1:209455/1‑71 (MQ=255) | CGGCTGACCAACATACTCTTCCAGTAATTTGGGGCGAATGGCGCGATCTGCTACATCTTCCGGCAAAGTGGTACCGGCAGAAAT > minE/1249168‑1249251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |