| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,266,244 | A→T | 100% | T127S (ACA→TCA) | tyrP → | tyrosine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,266,244 | 0 | A | T | 91.3% | 62.8 / ‑0.3 | 23 | T127S (ACA→TCA) | tyrP | tyrosine transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (0/21); total (1/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.70e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTGGCAGCATTGATTCAACAACCTTTATGGGATTGCTGGCTAATCATGCTGGATTAAACGGGCTGTTACAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGATT > minE/1266176‑1266308 | cTTGGCAGCATTGATTCAACAACCTTTATGGGATTGCTGGCTAATCATGCTGGATTAAACGGGCTGTTa > 1:363516/1‑69 (MQ=255) gATTCAACAACCTTTATGGGATTGCTGGCTAATCATGCTGGATTAAACGGGCTGTTACAGGCGTTAcgcg > 1:856182/1‑70 (MQ=255) cGGGCTGTTACAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTg < 1:1956048/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGTGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1143232/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:953081/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1040252/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:932227/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:796071/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:515343/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:434650/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:429167/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:2908333/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:2770084/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:2671581/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:2536444/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:248760/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:2161712/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1898817/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1896674/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1879609/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1456710/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1358454/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1211259/71‑1 (MQ=255) gCTGTTTCAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGAtt < 1:1208502/71‑1 (MQ=255) | CTTGGCAGCATTGATTCAACAACCTTTATGGGATTGCTGGCTAATCATGCTGGATTAAACGGGCTGTTACAGGCGTTACGCGAAATGGTGGCCTCTCCGCATGTTGAGCTGGCAGTGCATTTATTTGCTGATT > minE/1266176‑1266308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |