| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,511,656 | T→C | 100% | G308G (GGT→GGC) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,656 | 0 | T | C | 88.0% | 68.0 / 2.6 | 25 | G308G (GGT→GGC) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (22/0); total (24/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTC > minE/1511596‑1511701 | ggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATg > 1:1017238/1‑70 (MQ=255)ggCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATg > 1:2734380/1‑70 (MQ=255) ggCGTATAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:4512/1‑43 (MQ=38) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1032893/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:698921/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:669123/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:626162/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:605430/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:407298/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2878383/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2814880/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2759162/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2650753/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2576245/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2431401/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:2218364/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1799757/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1762886/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1634469/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1583537/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1509076/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1462409/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1366666/1‑43 (MQ=255) ggCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGCTACGaaa > 1:1359760/1‑43 (MQ=255) tgctgctgACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTc < 1:993881/62‑1 (MQ=255) | GGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTC > minE/1511596‑1511701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |