Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,532,388	T→A	100%	Q113L (CAA→CTA)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,532,388	0	T	A	100.0%	94.7 / NA	30	Q113L (CAA→CTA)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/0);  total (30/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACG  >  minE/1532356‑1532426                                 |                                       cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTaa                          >  1:1585833/1‑47 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1092176/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:847400/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:843605/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:654901/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:423926/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:305847/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2866666/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2788032/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:274165/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:269610/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2654859/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2557785/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2437999/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2400156/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2344045/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1867414/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1791131/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1718961/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1687197/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1634606/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1433154/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:141559/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1415266/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1327328/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1233632/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1166550/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:113885/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTAGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:1059084/1‑71 (MQ=255) cACCGCCGCGATAATCGCGCAGACCGTATTTTACTTCGTATGTGTAATCGGCATGGCCTGGACGGAAAACg  >  1:2201594/1‑71 (MQ=255)                                 |                                       CACCGCCGCGATAATCGCGCAGACCGTATTTTTGTTCGTAGGTGTAATCGGCATGGCCTGGACGGAAAACG  >  minE/1532356‑1532426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑