Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,587,552 C→T 100% G143G (GGC→GGT ptsI → PEP‑protein phosphotransferase of PTS system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,587,5520CT100.0% 11.2 / NA 5G143G (GGC→GGTptsIPEP‑protein phosphotransferase of PTS system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/0);  total (5/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGCCTGAAGATTATCGACCTGAGCGC  >  minE/1587505‑1587575
                                               |                       
gcagACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGa           >  1:254586/4‑62 (MQ=255)
gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc  >  1:1324616/1‑71 (MQ=255)
gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc  >  1:1944306/1‑71 (MQ=255)
gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc  >  1:2112979/1‑71 (MQ=255)
gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc  >  1:565842/1‑71 (MQ=255)
                                               |                       
GCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGCCTGAAGATTATCGACCTGAGCGC  >  minE/1587505‑1587575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: