| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,587,552 | C→T | 100% | G143G (GGC→GGT) | ptsI → | PEP‑protein phosphotransferase of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,587,552 | 0 | C | T | 100.0% | 11.2 / NA | 5 | G143G (GGC→GGT) | ptsI | PEP‑protein phosphotransferase of PTS system |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGCCTGAAGATTATCGACCTGAGCGC > minE/1587505‑1587575 | gcagACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGa > 1:254586/4‑62 (MQ=255)gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc > 1:1324616/1‑71 (MQ=255)gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc > 1:1944306/1‑71 (MQ=255)gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc > 1:2112979/1‑71 (MQ=255)gCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGTCTGAAGATTATCGACCTGAgcgc > 1:565842/1‑71 (MQ=255) | GCTGACGTACGTGATATCGGTAAGCGCCTGCTGCGCAACATCCTGGGCCTGAAGATTATCGACCTGAGCGC > minE/1587505‑1587575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |