Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,698,958 | A→G | 100% | I280V (ATT→GTT) | csiE → | stationary phase inducible protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,698,958 | 0 | A | G | 100.0% | 267.2 / NA | 69 | I280V (ATT→GTT) | csiE | stationary phase inducible protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/69); total (0/69) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGA > minE/1698911‑1698980 | ttaCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2182796/69‑1 (MQ=255) ataCCCTCCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:902490/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCTGGAaga < 1:373984/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:412825/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2232420/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2287797/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2325422/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:240966/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2444178/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2476274/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2679975/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2752148/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2814169/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2841448/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2904510/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:296842/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:223698/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:453537/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:521598/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:649608/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:655299/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:689741/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:703462/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:705186/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:713194/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:901297/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:940217/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:960492/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:990381/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:994684/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2104873/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1121795/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1131285/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1138595/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1224348/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1245273/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1293555/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1314960/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1336712/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1386927/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:14367/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1506596/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1561682/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1636594/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1747458/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1782633/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1819812/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2152440/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2095136/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2071438/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2044123/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2023859/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:198634/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1822855/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1817718/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:178782/70‑1 (MQ=255) ataCCCACCTCGCCCAGGCGTTAAATAGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:919091/70‑1 (MQ=255) ataCCCACATCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1020065/70‑1 (MQ=255) taCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1507785/69‑1 (MQ=255) taCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2723763/69‑1 (MQ=255) taCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:2823716/69‑1 (MQ=255) taCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1511691/69‑1 (MQ=255) taCCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:383287/69‑1 (MQ=255) cccACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:1299312/67‑1 (MQ=255) tCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:411287/61‑1 (MQ=255) cAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:272577/56‑1 (MQ=255) aGGCGTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:605351/55‑1 (MQ=255) gTTAAATCGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCCGGAaga < 1:15908/51‑1 (MQ=255) cGCAGTTTGTTTGCCATCGGTGTTGATAATACCCTGCAGGAaga < 1:2196345/44‑1 (MQ=37) | ATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGA > minE/1698911‑1698980 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |