| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,722,197 | A→T | 100% | intergenic (‑5/+160) | yfhG ← / ← yfhK | conserved hypothetical protein/predicted sensory kinase in two‑component system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,722,197 | 0 | A | T | 86.0% | 136.5 / 14.0 | 50 | intergenic (‑5/+160) | yfhG/yfhK | conserved hypothetical protein/predicted sensory kinase in two‑component system |
| Reads supporting (aligned to +/- strand): ref base A (0/7); new base T (0/43); total (0/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATG > minE/1722174‑1722251 | gTTGAAAAATGTGTCGCATATTTTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1146806/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2894176/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1967482/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1975145/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2221145/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:496277/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:337900/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2313262/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2902997/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2478762/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1910977/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2556457/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2888480/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2606713/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2626563/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2759478/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2807884/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2860359/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1635788/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1132447/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:992276/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1290422/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:956897/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1445221/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:899724/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1514284/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:850025/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:503450/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1656834/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:761952/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:74747/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:181892/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1827799/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:722048/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGAGCGTAAt < 1:1142818/56‑1 (MQ=255)gTTGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCACAGTGGGCGTAAt < 1:1774783/56‑1 (MQ=255) ttGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGTGCGTAAt < 1:1443404/55‑1 (MQ=255) ttGAAAAATGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:1506477/55‑1 (MQ=255) tGAAAAATGTGTCGCATATTCTCCAGACATAAAGCCTATCCCAGTGGGCGTAAt < 1:2919543/54‑1 (MQ=255) aaTGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:2411299/49‑1 (MQ=255) aaTGTGTCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:455109/49‑1 (MQ=255) aaTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:2515334/71‑1 (MQ=255) aaTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:2264815/71‑1 (MQ=255) aaTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:2263511/71‑1 (MQ=255) aaTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:1550200/71‑1 (MQ=255) aaTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:1152111/71‑1 (MQ=255) aaTGTGGCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:2603963/71‑1 (MQ=255) aTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtggacatg < 1:1870436/70‑1 (MQ=255) gtgtCGCATATTCTCCAGACTTAAAGCCTATCCCAGTGGGCGTAAt < 1:167737/46‑1 (MQ=255) atTCTCCAGACTTAAAGCCTAACCCAGTGGGCGTAAt < 1:1948880/37‑1 (MQ=38) | GTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATG > minE/1722174‑1722251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |