| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,386,361 | C→A | 100% | L189I (CTA→ATA) | metR → | DNA‑binding transcriptional activator, homocysteine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,386,361 | 0 | C | A | 100.0% | 39.0 / NA | 13 | L189I (CTA→ATA) | metR | DNA‑binding transcriptional activator, homocysteine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/13); total (0/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGCGAATTACACCGGAAGATCTCGCCAGCGAGACGCTATTAATTTATCCGGTGCAGCGTAGTCGACTGG > minE/2386325‑2386394 | aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1038575/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1089169/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1732674/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1798/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1904773/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:1954299/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:2396288/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:2430357/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:2570417/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:2673396/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:414657/70‑1 (MQ=255)aCGCGAATTACACCGGAAGATCTCGCCAGCGAGACGATATTAATTTATCCGGTGCAGCGTAGTCGACTgg < 1:893526/70‑1 (MQ=255) agaCGATTTTAATTTTTCCGGTGCAGCGTAGTCGACTgg < 1:1110681/39‑1 (MQ=25) | ACGCGAATTACACCGGAAGATCTCGCCAGCGAGACGCTATTAATTTATCCGGTGCAGCGTAGTCGACTGG > minE/2386325‑2386394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |