| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,397,027 | G→T | 100% | P54Q (CCG→CAG) | corA ← | magnesium/nickel/cobalt transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,397,027 | 0 | G | T | 78.7% | 76.2 / 32.9 | 47 | P54Q (CCG→CAG) | corA | magnesium/nickel/cobalt transporter |
| Reads supporting (aligned to +/- strand): ref base G (3/7); new base T (37/0); total (40/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGA > minE/2397007‑2397093 | cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:1906956/70‑1 (MQ=255)cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:1186217/70‑1 (MQ=255)cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:2225155/70‑1 (MQ=255)cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:1474023/70‑1 (MQ=255)cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:1531952/70‑1 (MQ=255)cTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCAGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:1229287/70‑1 (MQ=255)cTTCGATGTCTTCCAGCTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCtc < 1:705665/70‑1 (MQ=255) tCCTGGCGGGTTGCCAGTCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1035543/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGt > 1:417401/1‑48 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTGCGGTTCGa > 1:862620/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCg > 1:1994350/1‑69 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCg > 1:2532170/1‑69 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2915282/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2733227/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2533845/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:580461/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2357060/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2351681/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2292145/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2231548/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:845370/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2143678/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2096911/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:859289/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2027862/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:94754/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1577497/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1060912/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1124484/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1199397/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1406974/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1461351/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:152818/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1915759/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1579167/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1598105/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1708086/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:171619/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1720531/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1739515/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1764426/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1837562/1‑70 (MQ=255) tCCTGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1878284/1‑70 (MQ=255) tCCTGGCGGGTTGACAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:579656/1‑70 (MQ=255) tCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGt > 1:1111666/1‑48 (MQ=255) tCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:2859003/1‑70 (MQ=255) tCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGa > 1:1368237/1‑70 (MQ=255) | CTTCGATGTCTTCCAGTTCCGGGCGGGTTGCCAGGCTCTGGCCAAGTTCAGATTGTACGCGCAGTCGCTCGTCGTCGTCCGGTTCGA > minE/2397007‑2397093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |