| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,441,897 | C→T | 100% | L462L (TTG→TTA) | ilvA ← | threonine deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,441,897 | 0 | C | T | 100.0% | 14.4 / NA | 6 | L462L (TTG→TTA) | ilvA | threonine deaminase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/6); total (0/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAACAAAGAAATGTTCC > minE/2441840‑2441910 | aaGTTCGAACGCCTCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:1694610/71‑1 (MQ=255)aaGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:1189994/71‑1 (MQ=255)aaGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:1390019/71‑1 (MQ=255)aaGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:2157824/71‑1 (MQ=255)aaGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:2440867/71‑1 (MQ=255)aaGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCAAGGCTGCGATAGTGGAATAAAGAAATGTTcc < 1:1280079/71‑1 (MQ=255) | AAGTTCGAACGCCGCCAGTACGCGCCCGTAGTCGGTGCCATGGCTGCGATAGTGGAACAAAGAAATGTTCC > minE/2441840‑2441910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |