| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,639,808 | A→G | 25.0% | T245A (ACG→GCG) *147Q (TAA→CAA) |
ugpQ → yhhA ← |
glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,639,808 | 0 | A | G | 25.0% | 155.6 / 56.5 | 84 | T245A (ACG→GCG) *147Q (TAA→CAA) | ugpQ yhhA | glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (41/22); new base G (10/11); total (51/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGTT > minE/2639768‑2639874 | cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:796794/70‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:787841/70‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:670423/70‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:1804245/70‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:2674662/70‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgt < 1:434703/71‑2 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2887985/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1642300/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:186651/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2017018/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:208388/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2218422/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2323507/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2729773/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2309411/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:378922/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:461468/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:47930/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:608450/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:987873/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1312982/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1587334/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1553695/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1401519/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1218266/71‑1 (MQ=255)cATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:1196843/71‑1 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg > 1:311867/1‑48 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg > 1:1026200/1‑48 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg > 1:622766/1‑48 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg > 1:1062983/1‑48 (MQ=255) gCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAgg < 1:2452711/58‑1 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGttt > 1:1983461/1‑37 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGt > 1:1768194/1‑62 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2739209/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1376482/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1370057/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2686989/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1625752/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2892287/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:265842/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:299912/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1328770/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:494632/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:634459/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:674300/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:744040/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1131545/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:850014/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:985445/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1060313/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2062688/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1607285/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1721900/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1579664/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1871682/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1924792/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:194682/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1507703/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2218968/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2271125/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2368560/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2488405/1‑70 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1507353/1‑70 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAAt > 1:2670118/1‑46 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:287945/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:1292465/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:974000/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:1969385/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:1934040/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:1570505/1‑71 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCAGCGGCGTGCTGGGCTg > 1:1999564/1‑70 (MQ=255) gACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAAAGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:2400356/1‑71 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:1909322/70‑1 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:860150/70‑1 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:831282/70‑1 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:1366699/70‑1 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:234508/70‑1 (MQ=255) gAACTTTGCGGCCCAATAGTTTTCAACGGAATGGCAGGCTGCGGCGTGCTGGGCTGa < 1:2070592/57‑1 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:799504/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:2748740/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:2404268/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:979592/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:1553420/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTATCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGtt > 1:2612757/1‑71 (MQ=255) | CATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGTT > minE/2639768‑2639874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |