| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,927,411 | C→A | 100% | intergenic (‑76/‑191) | pepA ← / → yjgP | aminopeptidase A, a cyteinylglycinase/conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,927,411 | 0 | C | A | 100.0% | 11.2 / NA | 5 | intergenic (‑76/‑191) | pepA/yjgP | aminopeptidase A, a cyteinylglycinase/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTGG > minE/2927387‑2927456 | gCAAGCTTTCGTAACTCATGTCCGATGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTgg < 1:2057421/70‑1 (MQ=255)gCAAGCTTTCGTAACTCATGTCCGATGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTgg < 1:2350058/70‑1 (MQ=255)gCAAGCTTTCGTAACTCATGTCCGATGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTgg < 1:2908226/70‑1 (MQ=255)gCAAGCTTTCGTAACTCATGTCCGATGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTgg < 1:58894/70‑1 (MQ=255)gCAAGCTTTCGTAACTCATGTCCGATGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTgg < 1:618325/70‑1 (MQ=255) | GCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACGTTATTACGGCATTGG > minE/2927387‑2927456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |