Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,947,335	A→G	100%	L158P (CTG→CCG)	dnaT ←	DNA biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,947,335	0	A	G	100.0%	169.2 / NA	45	L158P (CTG→CCG)	dnaT	DNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/0);  total (45/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCG  >  minE/2947303‑2947374                                 |                                        gTTCGCTGACCGTATTCACATCTCGTTTGGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:314763/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTcc                                     >  1:962479/1‑37 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTg             >  1:1800786/1‑61 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1066518/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:528477/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:495542/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:471888/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2855413/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2617461/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2481103/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2381762/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2241625/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:2079976/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1465009/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1136016/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1097226/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1433708/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1294107/1‑71 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg    >  1:103340/1‑70 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg    >  1:2296617/1‑70 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg    >  1:1782397/1‑70 (MQ=255) gTTCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcg    >  1:177134/1‑70 (MQ=255)  ttagCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1155022/4‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGt                                >  1:1828377/1‑41 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:640573/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:609567/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1108418/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1130422/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:672370/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2904685/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:128046/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2770665/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2659974/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2549858/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1226274/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2379289/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2311088/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2257511/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1285418/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2176553/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:2139818/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:212807/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:156082/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgcg  >  1:1536391/1‑71 (MQ=255)  ttCGCTGACCGTATTCACATCTCGTTTCGGCGGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTgcgc   >  1:1201487/1‑70 (MQ=255)                                 |                                        GTTCGCTGACCGTATTCACATCTCGTTTCGGCAGTCCGCCGTTGCTGGCACGACCGATTTGCAGGCTGCGCG  >  minE/2947303‑2947374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑