| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 332,361 | G→A | 100% | L269L (CTG→TTG) | fsr ← | predicted fosmidomycin efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 332,361 | 0 | G | A | 81.5% | 219.7 / 41.6 | 92 | L269L (CTG→TTG) | fsr | predicted fosmidomycin efflux system |
| Reads supporting (aligned to +/- strand): ref base G (2/15); new base A (0/75); total (2/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCT > minE/332306‑332427 | ccTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCAtt > 1:2436621/1‑71 (MQ=255)ccTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCAt > 1:375263/1‑70 (MQ=255) tACCTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAtttt < 1:1461677/71‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:777277/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2874339/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:687879/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2789706/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2584489/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2508697/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2505648/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:70600/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2503684/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2884763/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:844450/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2411153/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2405397/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2339207/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2323989/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2320950/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2287876/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2278929/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:429231/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:646033/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:627059/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:618684/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:606234/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:603006/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:58458/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:52923/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:512466/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2878085/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:393359/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:34653/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:314809/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:299184/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2918174/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2917123/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:684559/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2230067/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1413707/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:86448/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1647487/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1632933/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:925102/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1588620/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:934715/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:149838/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1429229/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1649966/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1335781/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1238031/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1236280/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:951836/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1227653/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1204001/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:958962/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1018594/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:84639/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1743321/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1749965/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1813007/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1881144/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1885036/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2080866/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1889186/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1740929/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2066228/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:2010887/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1925440/70‑1 (MQ=255) ccTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTCGATAGATAATCCGAAttttt < 1:209451/70‑1 (MQ=255) ccTGCCACAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1299931/70‑1 (MQ=255) cTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1909644/69‑1 (MQ=255) cTGCCGCAACGGCAAACAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1307956/69‑1 (MQ=255) aCAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:84858/54‑1 (MQ=255) aCAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1862299/54‑1 (MQ=255) cAGGAAGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCTAAttttt < 1:730189/53‑1 (MQ=255) aGGCAAACAAATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:1037107/48‑1 (MQ=255) caaacaaaTGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt < 1:970084/45‑1 (MQ=255) aaCAGATGTAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAATGGTGTAATAgctgct < 1:80069/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:289339/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2503809/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:711445/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2495026/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2488391/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2481235/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2144842/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1912857/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1719689/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1627745/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1537684/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1231284/71‑1 (MQ=255) aaCAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:1108861/71‑1 (MQ=255) aaCAGATGAAGCTCAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgct < 1:2871765/71‑1 (MQ=255) | CCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCT > minE/332306‑332427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |