| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 52,517 | T→C | 23.5% | E757E (GAA→GAG) | imp ← | exported protein required for envelope biosynthesis and integrity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 52,517 | 0 | T | C | 23.5% | 92.2 / 29.7 | 51 | E757E (GAA→GAG) | imp | exported protein required for envelope biosynthesis and integrity |
| Reads supporting (aligned to +/- strand): ref base T (19/20); new base C (7/5); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GATACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTT > minE/52447‑52579 | gATACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGt > 1:990859/1‑71 (MQ=255) tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTc < 1:1654464/71‑1 (MQ=255) cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGa < 1:249028/71‑1 (MQ=255) gAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTa < 1:1725648/71‑1 (MQ=255) gAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGACGCGAAGTTCGATGTTa < 1:2003137/71‑1 (MQ=255) aTGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGAt > 1:1011762/1‑65 (MQ=255) aTGTTCGAACGCAGCATCTCTT‑CGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAg > 1:2039411/1‑71 (MQ=255) tCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGcc > 1:1681455/1‑70 (MQ=255) cGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGa > 1:2026833/1‑71 (MQ=255) cagcaTCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGt > 1:77385/1‑71 (MQ=255) cagcaTCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAg > 1:2073931/1‑61 (MQ=255) ctctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa > 1:78091/1‑70 (MQ=255) ctctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa > 1:2148871/1‑70 (MQ=255) tctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCata > 1:1432880/1‑71 (MQ=255) tctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCata > 1:1593832/1‑71 (MQ=255) tctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCata > 1:2150314/1‑71 (MQ=255) tctTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCat > 1:708360/1‑70 (MQ=255) ttGCGTACCCCGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa < 1:2182266/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCa < 1:1220242/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCa < 1:1689929/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCa < 1:1603001/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCAtata > 1:1111864/1‑71 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTGCGATGTTAAAGCCGATTGCGTTGTCa < 1:1398260/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa < 1:1962529/67‑1 (MQ=255) ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa < 1:1375538/67‑1 (MQ=255) gCGTACCCAGACCTTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCatat < 1:1174340/68‑1 (MQ=255) gCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATAcc > 1:1358985/1‑71 (MQ=255) gCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATAcc > 1:1083912/1‑71 (MQ=255) gCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATAcc > 1:1922944/1‑71 (MQ=255) cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAt < 1:35221/70‑1 (MQ=255) cAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGcc < 1:656561/45‑1 (MQ=255) cAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGcc < 1:2121760/45‑1 (MQ=255) cAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCAtata > 1:1009020/1‑62 (MQ=255) cAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgtt > 1:473129/1‑71 (MQ=255) aGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgttg > 1:2013794/1‑71 (MQ=255) ccGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATgttgtt > 1:1369464/1‑70 (MQ=255) cGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCat < 1:1609521/54‑1 (MQ=255) cGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTa < 1:2212623/71‑1 (MQ=255) cGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTa < 1:243400/71‑1 (MQ=255) gTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGc < 1:951658/42‑1 (MQ=255) gTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGt < 1:1118631/71‑1 (MQ=255) gTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGt < 1:2099909/71‑1 (MQ=255) ggAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTAt > 1:581260/1‑71 (MQ=255) ggAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTAt > 1:1910183/1‑71 (MQ=255) ggAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATAC‑‑CATGTTGTTTATCGTTATc < 1:1315466/70‑1 (MQ=255) gAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTAt < 1:819143/70‑1 (MQ=255) gCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgttgt < 1:1396922/52‑1 (MQ=255) gCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgt < 1:2213139/49‑1 (MQ=255) ccGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt > 1:1870559/1‑71 (MQ=255) ccGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt > 1:1504248/1‑71 (MQ=255) ccGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt > 1:1896067/1‑71 (MQ=255) cgcgAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt > 1:226993/1‑70 (MQ=255) | GATACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTT > minE/52447‑52579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |