| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 297,667 | C→T | 26.7% | R110C (CGT→TGT) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 297,667 | 0 | C | T | 26.7% | 75.6 / 28.8 | 45 | R110C (CGT→TGT) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (7/5); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01 | |||||||||||
TCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAG > minE/297597‑297736 | tCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGc < 1:725832/71‑1 (MQ=255) aaCTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGATTTACCTgtgt < 1:357714/71‑1 (MQ=255) cTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:122450/71‑1 (MQ=255) cTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGc < 1:1517844/71‑1 (MQ=255) cTGTTGAACTGCGCGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGc < 1:1464616/71‑1 (MQ=255) tGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGc > 1:1279486/1‑70 (MQ=255) gTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTc < 1:412087/71‑1 (MQ=255) gTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTc < 1:2112048/71‑1 (MQ=255) gTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTc < 1:1755810/71‑1 (MQ=255) aaGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATg > 1:2073490/1‑71 (MQ=255) aGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATgg > 1:2171321/1‑71 (MQ=255) aGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATgg > 1:1484429/1‑71 (MQ=255) tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCg > 1:530903/1‑71 (MQ=255) attaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGt > 1:984176/1‑71 (MQ=255) ttaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCa < 1:393442/45‑1 (MQ=255) aCCGTCAGCTAAGCCGTCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGc > 1:1703250/1‑70 (MQ=255) aCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGc < 1:1714432/70‑1 (MQ=255) ccGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCg < 1:481373/70‑1 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt > 1:1569497/1‑71 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCCCCGGTGACCTc > 1:1349064/1‑53 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCa > 1:753769/1‑54 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt < 1:1461208/71‑1 (MQ=255) aGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAAt < 1:1139932/71‑1 (MQ=255) cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCt < 1:1357072/50‑1 (MQ=255) cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCt < 1:1217780/50‑1 (MQ=255) cTAAGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCt < 1:1327576/50‑1 (MQ=255) tAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATg > 1:2213514/1‑69 (MQ=255) aaGCCGGCAGCATCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACg > 1:113958/1‑71 (MQ=255) aaGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCaa > 1:730576/1‑66 (MQ=255) ccGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg < 1:1900166/71‑1 (MQ=255) cGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCg < 1:2209705/70‑1 (MQ=255) agcaTCCTGAGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATcgc > 1:767033/1‑71 (MQ=255) agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTgc > 1:1832428/1‑36 (MQ=255) agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATcgc > 1:1040845/1‑71 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATGGCGTCgtg > 1:1133366/1‑71 (MQ=255) cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtg > 1:480308/1‑71 (MQ=255) gAGTTTTACCTGTGTCATCGCACAGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGtt > 1:1216767/1‑71 (MQ=255) gAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGtt > 1:1342204/1‑71 (MQ=255) aGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtgt < 1:343587/69‑1 (MQ=255) aGTTTTACCTGTGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGttt > 1:1428988/1‑71 (MQ=255) aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtgt < 1:2048310/69‑1 (MQ=255) aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCgtgt < 1:1743837/69‑1 (MQ=255) aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGttt > 1:832041/1‑71 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGcgtcgt < 1:186275/65‑1 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATGGATCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgc > 1:1328617/1‑71 (MQ=255) ttttACCTGCGTCATCGCACCGGTGACCTCATCGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgc > 1:2005287/1‑71 (MQ=255) ccTGCGTCATCGCACCGGTTACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTgccgc < 1:969847/69‑1 (MQ=255) cGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGgag < 1:1690423/70‑1 (MQ=255) cGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGgac < 1:1275967/70‑2 (MQ=255) | TCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAG > minE/297597‑297736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |