| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,269,809 | T→C | 9.4% | V174V (GTA→GTG) | murB ← | UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,269,809 | 0 | T | C | 9.4% | 129.2 / 7.3 | 53 | V174V (GTA→GTG) | murB | UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base T (18/30); new base C (3/2); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCCAGACGAGTTAAGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCACTCTTT > minE/2269741‑2269877 | tCCAGACGAGTTAAGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTAc < 1:1162405/71‑1 (MQ=255) cAGACGAGTTAAGTCACCATACGTTAGTTCAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACaa > 1:2120001/1‑71 (MQ=255) cAGACGAGTTAAGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACaa > 1:316679/1‑71 (MQ=255) ttAAGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTAc < 1:1913185/61‑1 (MQ=255) tAAGTCACCATACGTTAGTACAGGTTGCCCCTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGc > 1:543580/1‑71 (MQ=255) aaGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACaa > 1:1127223/1‑61 (MQ=255) aaGTCACCATACGTTAGTACAGGTTGACACTCTTTTGGCAGACGCAGACCTACGGCTACaa > 1:788799/1‑61 (MQ=255) aGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCgg < 1:1660962/71‑1 (MQ=255) ccATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTcc < 1:1831906/69‑1 (MQ=255) cATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTgg > 1:2152999/1‑71 (MQ=255) tACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTAc < 1:933434/50‑1 (MQ=255) tACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTAc < 1:768063/50‑1 (MQ=255) tACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTAc < 1:2172883/50‑1 (MQ=255) cGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTAtt < 1:901486/71‑1 (MQ=255) cGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTAtt < 1:35342/71‑1 (MQ=255) gTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTg < 1:210099/65‑1 (MQ=255) tAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGt > 1:1780342/1‑59 (MQ=255) tAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCAt > 1:602060/1‑71 (MQ=255) tACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTgg < 1:1774395/61‑1 (MQ=255) ggTTGCCACTCTTTTGGCAGACTCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaa < 1:57952/71‑1 (MQ=255) gTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaa < 1:294916/70‑1 (MQ=255) tGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaaa > 1:1615758/1‑70 (MQ=255) gCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAAt < 1:2152537/70‑1 (MQ=255) gCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAAt < 1:1515897/70‑1 (MQ=255) aCTCTTTTGGCAGACGCAGACCTACGGCCACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTg > 1:1797702/1‑71 (MQ=255) aCTCATTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTc > 1:1419284/1‑46 (MQ=255) tctTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGc < 1:158214/40‑1 (MQ=255) tctTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGt < 1:1692195/43‑1 (MQ=255) ctTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTcg > 1:1354044/1‑71 (MQ=255) ctTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTcg > 1:432959/1‑71 (MQ=255) ttttGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTgg < 1:1650736/46‑1 (MQ=255) ggCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGAt < 1:404931/70‑1 (MQ=255) gcagacgcagacCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATa < 1:982463/70‑1 (MQ=255) gcagacgcagacCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATa < 1:1664188/70‑1 (MQ=255) acgcagacCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaaa > 1:772030/1‑53 (MQ=255) acgcagacCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa < 1:825173/71‑1 (MQ=255) cgcagacCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa > 1:1365107/1‑70 (MQ=255) cgcagacCTACGGCCACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa > 1:1563924/1‑70 (MQ=255) cgcagacCTACGGCCACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa > 1:1163250/1‑70 (MQ=255) acCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTcgc < 1:1230333/56‑1 (MQ=255) acCTACGGCCACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa < 1:2031096/65‑1 (MQ=255) acCTACGGCCACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCaa < 1:923517/65‑1 (MQ=255) ccTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACggg > 1:1721842/1‑69 (MQ=255) cTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGtt < 1:1598509/39‑1 (MQ=255) cTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaa < 1:520919/43‑1 (MQ=255) cTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCAc > 1:1214168/1‑71 (MQ=255) cTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCAc > 1:1027255/1‑71 (MQ=255) aCGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCActc > 1:164471/1‑71 (MQ=255) cGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaaa < 1:1510667/42‑1 (MQ=255) gCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaa < 1:482810/39‑1 (MQ=255) gCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCACTCttt < 1:1469654/71‑1 (MQ=255) cTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaaa < 1:854728/39‑1 (MQ=255) cTACAATAGCGAAGCGGTCCTGGTATTCATGTTTaaaaa < 1:1208624/39‑1 (MQ=255) | TCCAGACGAGTTAAGTCACCATACGTTAGTACAGGTTGCCACTCTTTTGGCAGACGCAGACCTACGGCTACAATAGCGAAGCGGTCCTGGTATTCATGTTTAAAAATACTGTCGCGATAGCCAAAACGGCACTCTTT > minE/2269741‑2269877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |