| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,283,783 | G→T | 14.2% | L39M (CTG→ATG) | oxyR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,283,783 | 0 | G | T | 14.2% | 143.4 / 6.5 | 49 | L39M (CTG→ATG) | oxyR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (21/21); new base T (3/4); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAT > minE/2283714‑2283848 | ttCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:1682939/1‑71 (MQ=255) cccGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCt > 1:1638856/1‑70 (MQ=255) gCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTAc > 1:398796/1‑70 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:1179213/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:1551630/71‑1 (MQ=255) cTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:102755/71‑1 (MQ=255) gggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACg < 1:1485762/67‑1 (MQ=255) gggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACg < 1:1691707/67‑1 (MQ=255) gggTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa < 1:1238530/69‑1 (MQ=255) acaacaCTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCg < 1:569865/71‑1 (MQ=255) acacTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCt > 1:2029710/1‑70 (MQ=255) acTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGc > 1:1281624/1‑51 (MQ=255) acTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAg > 1:45023/1‑71 (MQ=255) cTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGc > 1:1673220/1‑71 (MQ=255) tttAAGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCg > 1:2136686/1‑71 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGaa > 1:1837849/1‑54 (MQ=255) tACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAtt > 1:640498/1‑56 (MQ=255) ctgCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCg < 1:1887100/68‑1 (MQ=255) cGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCg < 1:9633/70‑1 (MQ=255) tGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACg < 1:962364/46‑1 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAAtt > 1:217586/1‑45 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTaa < 1:197629/70‑1 (MQ=255) cGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAAc < 1:1547913/71‑1 (MQ=255) gCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACg < 1:737376/71‑1 (MQ=255) cTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGt < 1:199313/71‑1 (MQ=255) cAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTgg < 1:1894254/70‑1 (MQ=255) gCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGc > 1:103891/1‑41 (MQ=255) aaCATCACGCCCAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGa < 1:1316241/71‑1 (MQ=255) aaCATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAgg > 1:1009171/1‑70 (MQ=255) aaCATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGa < 1:1286993/71‑1 (MQ=255) cATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTaa < 1:1878675/44‑1 (MQ=255) aTCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATc > 1:1536426/1‑71 (MQ=255) cACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTg > 1:2197826/1‑71 (MQ=255) aCGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGc > 1:798452/1‑71 (MQ=255) cccAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCg > 1:983111/1‑70 (MQ=255) ccAGCTCATCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCa < 1:295551/71‑1 (MQ=255) gCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAgg < 1:1884211/57‑1 (MQ=255) cTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAgg < 1:42843/56‑1 (MQ=255) cTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAgg < 1:1450385/56‑1 (MQ=255) cATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCg > 1:1997078/1‑70 (MQ=255) aTCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGa < 1:1109367/70‑1 (MQ=255) tCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTgg < 1:1983665/48‑1 (MQ=255) tCTTCCATCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGAACGCCGaa > 1:347989/1‑70 (MQ=255) cTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGaaaa > 1:906740/1‑71 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTaa > 1:1928930/1‑41 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAAt > 1:1936503/1‑53 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAAt > 1:700910/1‑53 (MQ=255) ttCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAt > 1:839558/1‑71 (MQ=255) cAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGaaa < 1:1597717/66‑1 (MQ=255) | TTCCCGCCTGGGTGAACAACACTTTACGGCTGGTCCGCTCCAGCAACATCACGCCCAGCTCATCTTCCAGCTTACGAATTTGCCCGCTAAGCGTCGGCTGGCTAACGTGGCAGGAATCTGCCGCACGCCGAAAAT > minE/2283714‑2283848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |