| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,321,698 | G→A | 13.4% | T93I (ACC→ATC) | cdh ← | CDP‑diacylglycerol phosphotidylhydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,321,698 | 0 | G | A | 13.4% | 141.6 / 12.3 | 52 | T93I (ACC→ATC) | cdh | CDP‑diacylglycerol phosphotidylhydrolase |
| Reads supporting (aligned to +/- strand): ref base G (11/34); new base A (3/4); total (14/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
TTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATTAA > minE/2321634‑2321767 | ttttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATca > 1:643831/1‑68 (MQ=255) tttttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTcaaca > 1:597777/1‑70 (MQ=255) ttGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAg < 1:1055227/70‑1 (MQ=255) cTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACt < 1:868845/71‑1 (MQ=255) cATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTc < 1:1898195/58‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAg < 1:1994996/64‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAg < 1:364095/64‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAg < 1:1186693/64‑1 (MQ=255) aTAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACttt > 1:527648/1‑70 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTcaaca < 1:311917/60‑1 (MQ=255) tAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTc < 1:1484017/70‑1 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGt < 1:634787/71‑1 (MQ=255) aaaTCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGt < 1:1878162/71‑1 (MQ=255) gcCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAg > 1:1548325/1‑61 (MQ=255) cTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:246963/71‑1 (MQ=255) cTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatac < 1:1687463/71‑1 (MQ=255) tGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac > 1:1642558/1‑70 (MQ=255) ccaagccaaCCAAAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAatacga > 1:558970/1‑70 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga < 1:732519/65‑1 (MQ=255) ccaaccaaAAGAAGTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:1125281/71‑1 (MQ=255) accaaAAGAAGTTCGGCGTTGAAGGATCGGTcaac < 1:180962/35‑1 (MQ=255) aaaaGAAGTTCGGCGTTGAAGGATCTATCAACAAAGGACTTTCAGTACCGTTAatacgatac < 1:1027673/62‑1 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:1920885/64‑1 (MQ=255) aaaGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGtt < 1:2149632/64‑1 (MQ=255) aagaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa < 1:1283887/71‑1 (MQ=255) aagaagTTCGGCGTTGAAGGATCGATCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa < 1:573516/71‑1 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGa > 1:1592139/1‑35 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTAcc > 1:311565/1‑46 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTAcc > 1:2008223/1‑46 (MQ=255) agaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:1839810/1‑70 (MQ=255) gaagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacga < 1:1895565/55‑1 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatacg > 1:870667/1‑53 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCaa > 1:1376627/1‑68 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:536455/71‑1 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:1016894/71‑1 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:478916/71‑1 (MQ=255) aagTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAg < 1:239706/71‑1 (MQ=255) agTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:302115/71‑1 (MQ=255) agTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:433389/71‑1 (MQ=255) gTTCGGCGTTGAAGGATCGGTCAACAACGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:1464055/70‑1 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:186460/71‑1 (MQ=255) ggCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTg < 1:1506270/71‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAatac < 1:1086514/42‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:1805898/62‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:2078006/62‑1 (MQ=255) ttGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATAAGATACGTTGGCATCAACAGATATTGCAGt < 1:647538/71‑1 (MQ=255) gAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCa > 1:2115089/1‑52 (MQ=255) gATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGc < 1:530885/47‑1 (MQ=255) gATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGc < 1:182680/47‑1 (MQ=255) gATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGc < 1:692898/47‑1 (MQ=255) gATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGc < 1:7059/47‑1 (MQ=255) tCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACa > 1:1185462/1‑52 (MQ=255) ggTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAGCAGATATTGCAGTGGGCCATTaa < 1:1938018/71‑1 (MQ=255) ggTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCAtt > 1:1186865/1‑69 (MQ=255) ggTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATTaa < 1:1563513/71‑1 (MQ=255) ggTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATTaa < 1:1824114/71‑1 (MQ=255) gTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGa < 1:715948/51‑1 (MQ=255) | TTTTTTGCTCATAAAATCACGCGCCTGCCAAGCCAACCAAAAGAAGTTCGGCGTTGAAGGATCGGTCAACAAAGGACTTTCAGTACCGTTAATACGATACGTTGGCATCAACAGATATTGCAGTGGGCCATTAA > minE/2321634‑2321767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |