| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,364,103 | A→G | 30.6% | W456R (TGG→CGG) | trkH ← | potassium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,364,103 | 0 | A | G | 30.6% | 96.0 / 62.0 | 72 | W456R (TGG→CGG) | trkH | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base A (38/12); new base G (16/6); total (54/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGGGTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTATTCAA > minE/2364034‑2364172 | tCGGGGTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCAt > 1:168398/1‑71 (MQ=255) gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCAt > 1:1904397/1‑66 (MQ=255) aaGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCAc > 1:1206171/1‑71 (MQ=255) gagCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCAcag > 1:734614/1‑69 (MQ=255) gagCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCg > 1:697443/1‑71 (MQ=255) agCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGttt > 1:199421/1‑62 (MQ=255) aCCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGtt < 1:7610/71‑1 (MQ=255) aCCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGtt < 1:431190/71‑1 (MQ=255) aGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCa > 1:32400/1‑70 (MQ=255) gCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATa > 1:437653/1‑71 (MQ=255) tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa < 1:1844005/63‑1 (MQ=255) tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCAc > 1:2144879/1‑55 (MQ=255) tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGt > 1:128868/1‑70 (MQ=255) tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGt > 1:1984893/1‑70 (MQ=255) tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGt > 1:655905/1‑70 (MQ=255) aaaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc > 1:1597588/1‑65 (MQ=255) aaaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc > 1:2082774/1‑65 (MQ=255) aaaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc > 1:69099/1‑65 (MQ=255) aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCg > 1:2045819/1‑55 (MQ=255) aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTaa < 1:1746046/70‑1 (MQ=255) ccTCGAGACGACCAAACAGGATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa > 1:1418591/1‑71 (MQ=255) ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGtt < 1:664585/70‑1 (MQ=255) cTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTAt < 1:2177975/71‑1 (MQ=255) tCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCa < 1:872664/55‑1 (MQ=255) tCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTAt > 1:542139/1‑70 (MQ=255) cGAGACGACCAAACAGCATTTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTAt > 1:213754/1‑69 (MQ=255) cGAGACGACCAAACAGCATGTTGGCTATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt > 1:1873776/1‑69 (MQ=255) cGAGACGACCAAACAGCATGTTGGCAATCAGGATCCa > 1:1510549/1‑37 (MQ=255) cGAGACGACCAAACAGCATGTTGGCAATCAGGATCCa > 1:1329063/1‑37 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCa > 1:1826301/1‑36 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGcc > 1:704592/1‑43 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCa > 1:876864/1‑44 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCa > 1:445148/1‑44 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCa > 1:587074/1‑44 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAg > 1:1787535/1‑71 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAg > 1:1513211/1‑71 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAg > 1:1390623/1‑71 (MQ=255) gagaCGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAg > 1:1187583/1‑71 (MQ=255) gagaCGACCAAACAGCATGTTGGCAAT‑AGGATCCATTTAGCCa > 1:563203/1‑43 (MQ=39) gacgacCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGc > 1:770810/1‑70 (MQ=255) gacgacCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATa > 1:699152/1‑53 (MQ=255) gacgacAAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATa > 1:2041533/1‑53 (MQ=255) gacCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAAc > 1:2082501/1‑70 (MQ=255) gacCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCAcc > 1:965772/1‑41 (MQ=255) gacCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTAt > 1:1285847/1‑63 (MQ=255) gacCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAAc > 1:160243/1‑70 (MQ=255) acCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa > 1:1031799/1‑71 (MQ=255) acCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCAt > 1:668436/1‑48 (MQ=255) aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGtt < 1:2029522/41‑1 (MQ=255) aCAGCATGTTGGCAATCATGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGcc > 1:1515178/1‑70 (MQ=255) aCAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGt > 1:769870/1‑54 (MQ=255) aCAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGcc > 1:1383975/1‑70 (MQ=255) cAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCaa < 1:792130/71‑1 (MQ=255) cAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCaa < 1:1464080/71‑1 (MQ=255) aGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa > 1:1555111/1‑40 (MQ=255) aGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa > 1:1382246/1‑40 (MQ=255) aGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATa > 1:119751/1‑42 (MQ=255) aGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa > 1:1946804/1‑64 (MQ=255) aGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa > 1:654342/1‑64 (MQ=255) gCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTgg < 1:869620/45‑1 (MQ=255) cATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGcc < 1:1191652/71‑1 (MQ=255) tGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCt < 1:1227617/71‑1 (MQ=255) gTTGGCAATCAGGATCCATTTAGCCACCGGGTTCa > 1:718146/1‑35 (MQ=255) ttGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTgg > 1:284300/1‑71 (MQ=255) ttGGCAATCAGGATCCATTTAGCCACCGGGTTCATAc < 1:1954051/37‑1 (MQ=255) gCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc > 1:1600469/1‑71 (MQ=255) aTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAg < 1:615831/59‑1 (MQ=255) cAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTAt > 1:1894694/1‑41 (MQ=255) aGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGt < 1:153649/70‑1 (MQ=255) aGGATCCATTTAGCCTCCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGt < 1:1791919/70‑1 (MQ=255) gATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAt < 1:1155677/71‑1 (MQ=255) gATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTa > 1:707664/1‑70 (MQ=255) aTCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGcccc > 1:1823528/1‑63 (MQ=255) tCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAtt > 1:1553981/1‑70 (MQ=255) ccATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTATTc > 1:1518505/1‑70 (MQ=255) aTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTATTCaa > 1:1450879/1‑70 (MQ=255) | TCGGGGTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTATTCAA > minE/2364034‑2364172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |