| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,371,447 | A→G | 48.6% | F214L (TTT→CTT) | fre ← | flavin reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,371,447 | 0 | A | G | 48.6% | ‑4.7 / 51.1 | 35 | F214L (TTT→CTT) | fre | flavin reductase |
| Reads supporting (aligned to +/- strand): ref base A (10/8); new base G (12/5); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
TTATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCGT > minE/2371378‑2371513 | ttATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCaaaa < 1:840425/70‑1 (MQ=255)ttATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCaaaa < 1:402383/70‑1 (MQ=255) aaaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGTCaa > 1:1876180/1‑71 (MQ=255) aaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGAt > 1:1600989/1‑61 (MQ=255) aaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCaa < 1:89021/70‑1 (MQ=255) cGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATcgc > 1:1709547/1‑56 (MQ=255) gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCa < 1:921216/70‑1 (MQ=255) gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCa > 1:1400406/1‑70 (MQ=255) aaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCa < 1:1045748/40‑1 (MQ=255) aaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCAATCTCAAAACg < 1:1390072/71‑1 (MQ=255) aCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGt > 1:1159776/1‑71 (MQ=255) cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc < 1:1817885/71‑1 (MQ=255) cTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAAc > 1:2173836/1‑60 (MQ=255) ttCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc > 1:2113936/1‑59 (MQ=255) ttCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCaa < 1:742400/56‑1 (MQ=255) cccGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCaa > 1:666105/1‑66 (MQ=255) cccGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCaa < 1:2028684/66‑1 (MQ=255) aTTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGat < 1:437924/66‑1 (MQ=255) aTTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGat < 1:1228545/66‑1 (MQ=255) aTTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCAt > 1:798897/1‑71 (MQ=255) ttACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATTTCAAAACGTCCGGCAAtata < 1:46472/62‑1 (MQ=255) aCGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa > 1:1773079/1‑62 (MQ=255) aCGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa > 1:1157345/1‑62 (MQ=255) aCGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa > 1:764244/1‑62 (MQ=255) aCGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa > 1:141185/1‑62 (MQ=255) gCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct < 1:462353/71‑1 (MQ=255) gCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct < 1:1061265/71‑1 (MQ=255) aCTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGc > 1:450345/1‑69 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaaa > 1:850635/1‑36 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaaa > 1:1704019/1‑36 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaaa > 1:410261/1‑36 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaaa > 1:199482/1‑36 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCaaaa > 1:1685523/1‑36 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc > 1:39075/1‑40 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc > 1:1267612/1‑40 (MQ=255) cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc > 1:1378595/1‑40 (MQ=255) cAAAACAGATCGCGGGCAATTTTCTCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCGt < 1:1255640/71‑1 (MQ=255) aaaCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAg < 1:1501982/49‑1 (MQ=255) aaaCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAg < 1:1847823/49‑1 (MQ=255) | TTATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCGT > minE/2371378‑2371513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |