| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,377,802 | A→T | 20.0% | Y196N (TAC→AAC) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,377,802 | 0 | A | T | 20.0% | 49.9 / 9.3 | 25 | Y196N (TAC→AAC) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base A (9/11); new base T (2/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
TTGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGAG > minE/2377732‑2377867 | ttGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTa > 1:2098347/1‑71 (MQ=255) gcgcCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACt > 1:2041645/1‑71 (MQ=255) cgcCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTc < 1:2081741/71‑1 (MQ=255) aTGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:1863551/71‑1 (MQ=255) ggCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTggg < 1:379516/70‑1 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGgcgc < 1:2184799/71‑1 (MQ=255) cAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGgcgc < 1:2115537/71‑1 (MQ=255) aGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGgcgc < 1:898756/70‑1 (MQ=255) aGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCa < 1:1328019/71‑1 (MQ=255) aGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCa < 1:1133777/71‑1 (MQ=255) tCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACg > 1:826350/1‑71 (MQ=255) tCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGa > 1:1454965/1‑69 (MQ=255) cagcaAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGAc < 1:106209/71‑1 (MQ=255) gcaAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCa > 1:67880/1‑71 (MQ=255) aaaTTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTg > 1:1770164/1‑52 (MQ=255) aTTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:1953329/45‑1 (MQ=255) aTTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTc < 1:76808/45‑1 (MQ=255) tCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCgg > 1:373538/1‑45 (MQ=255) tCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCgg > 1:1180219/1‑45 (MQ=255) ttCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTgg < 1:146359/45‑1 (MQ=255) aatcaatGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAAcgcg > 1:162744/1‑71 (MQ=255) tcaatGTCTTTTCGTACTCGCGCACCACTTCGGtt > 1:759873/1‑35 (MQ=255) tCTTTTCGTACTCGCTCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAAcg < 1:1682868/61‑1 (MQ=255) ttttCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAAcgcg > 1:692672/1‑61 (MQ=255) ttCGTTCTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGAg > 1:2117246/1‑71 (MQ=255) tCGTACTCGCGCACCACTTCGGGTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCg < 1:786772/68‑1 (MQ=255) | TTGCGCCGAAGCTGAATGGCGTTGGCAGATTCCCGCAGCAAATTCAGTTCATCAATCAATGTCTTTTCGTACTCGCGCACCACTTCGGTTGGGCGCAGACGGCGACCATCCGGCAGCAAACGCGGCACCCAGCGAG > minE/2377732‑2377867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |