| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,450,298 | A→G | 22.2% | W78R (TGG→CGG) | yifE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,450,298 | 0 | A | G | 22.2% | 82.2 / 24.0 | 45 | W78R (TGG→CGG) | yifE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (19/16); new base G (6/4); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 | |||||||||||
ACCTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTCCTC > minE/2450228‑2450367 | aCCTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACca < 1:288825/71‑1 (MQ=255) tGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCgcac > 1:1983996/1‑71 (MQ=255) ggTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACAcgcg < 1:1224786/71‑1 (MQ=255) ggTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACAcgcg < 1:1227252/71‑1 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt < 1:1742132/71‑1 (MQ=255) ttACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTc > 1:865472/1‑71 (MQ=255) ttACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTc > 1:1643955/1‑71 (MQ=255) tACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCt > 1:1889395/1‑71 (MQ=255) ccgccgGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGCCCACACGCGTTCTGc > 1:635406/1‑71 (MQ=255) ggAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTg > 1:295147/1‑71 (MQ=255) aaGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCa > 1:1423847/1‑71 (MQ=255) gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcac > 1:907518/1‑48 (MQ=255) tgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTc < 1:2117600/71‑1 (MQ=255) tgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTc < 1:1451253/71‑1 (MQ=255) tgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGt > 1:842358/1‑51 (MQ=255) tgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTc > 1:61730/1‑59 (MQ=255) gAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCa > 1:143374/1‑71 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:2126602/1‑71 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:442841/1‑71 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:796128/1‑71 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:184510/1‑71 (MQ=255) aaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGt < 1:327112/71‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1703472/70‑1 (MQ=255) gACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACgg < 1:740765/71‑1 (MQ=255) aCGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGc < 1:2163454/71‑1 (MQ=255) aCGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGc < 1:301457/71‑1 (MQ=255) cGCTTAATACGCGTCATATACTTGGACCACAcgcg < 1:1234381/35‑1 (MQ=255) ttAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGt > 1:187892/1‑57 (MQ=255) ttAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGt > 1:266182/1‑57 (MQ=255) tAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:954258/63‑1 (MQ=255) aaTACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTc < 1:226029/43‑1 (MQ=255) aTACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGc > 1:970498/1‑71 (MQ=255) aTACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGc < 1:1021187/71‑1 (MQ=255) aTACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGc < 1:354822/71‑1 (MQ=255) aTACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGc > 1:1933489/1‑71 (MQ=255) tACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGt < 1:883447/40‑1 (MQ=255) aCGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACt > 1:1948201/1‑43 (MQ=255) atatACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc > 1:638525/1‑52 (MQ=255) tataCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGt > 1:741195/1‑71 (MQ=255) ataCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:612592/54‑1 (MQ=255) taCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCg > 1:19166/1‑45 (MQ=255) taCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCg > 1:812470/1‑45 (MQ=255) taCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCg > 1:230607/1‑45 (MQ=255) taCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:1941665/1‑71 (MQ=255) taCTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:263402/1‑70 (MQ=255) ttGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGt < 1:2128979/66‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct < 1:48376/70‑1 (MQ=255) ggACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:150615/69‑1 (MQ=255) cacaCGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:399534/44‑1 (MQ=255) cacaCGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:1576301/44‑1 (MQ=255) cacaCGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:363503/44‑1 (MQ=255) cacaCGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:1532006/44‑1 (MQ=255) cacaCGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTCCTc < 1:1756620/71‑1 (MQ=255) | ACCTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTCCTC > minE/2450228‑2450367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |