| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 298,537 | C→T | 27.9% | L400L (CTG→TTG) | mdlA → | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 298,537 | 0 | C | T | 27.9% | 57.8 / 23.4 | 37 | L400L (CTG→TTG) | mdlA | fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (12/14); new base T (6/4); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTT > minE/298474‑298604 | ttgtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGa < 1:159724/70‑1 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGt > 1:764720/1‑71 (MQ=255) gtCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGt > 1:743583/1‑71 (MQ=255) gCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:865796/71‑1 (MQ=255) tCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACaa > 1:975208/1‑71 (MQ=255) ttCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:1272856/66‑1 (MQ=255) cAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGa > 1:1598020/1‑71 (MQ=255) tCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCg < 1:1439834/65‑1 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACa > 1:472783/1‑58 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGtt < 1:183177/55‑1 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGtt < 1:2214117/55‑1 (MQ=255) aTTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTgg > 1:2014656/1‑71 (MQ=255) ttCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCg > 1:615635/1‑71 (MQ=255) cGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGt < 1:1153906/70‑1 (MQ=255) tCAGCGAGGGGGATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTc < 1:1255983/53‑1 (MQ=255) gCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAgc > 1:2091663/1‑65 (MQ=255) aGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCg > 1:382521/1‑71 (MQ=255) gggggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCgg < 1:936595/71‑1 (MQ=255) ggggATATTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGt < 1:1682024/71‑1 (MQ=255) ggggATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCgg < 1:1118913/70‑1 (MQ=255) ggATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:352223/38‑1 (MQ=255) ggATATTCGCTTTCATGATATTCCTCTGACGAAGTTAc < 1:806463/38‑1 (MQ=255) atatTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTAGATAGCTGGCGTAGCCGCCTGGCGGTAGtt < 1:545395/71‑1 (MQ=255) tatTCGCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTa < 1:1070032/71‑1 (MQ=255) atTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAg > 1:539779/1‑71 (MQ=255) ttCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGa < 1:1425895/40‑1 (MQ=255) cGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAg > 1:321494/1‑64 (MQ=255) cGCTTTCATGATATTCCTATGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTa > 1:381793/1‑63 (MQ=255) gCTTTCATGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTgg > 1:125594/1‑57 (MQ=255) gCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCa < 1:944080/70‑1 (MQ=255) aTGATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGa > 1:1177176/1‑66 (MQ=255) gATATTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCAtt > 1:2154150/1‑71 (MQ=255) atatTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTc > 1:1189493/1‑71 (MQ=255) tatTCCTTTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCa < 1:655250/67‑1 (MQ=255) tCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCttt > 1:194211/1‑71 (MQ=255) ccTTTGACGAAGTTACAACTCGATAGCTGGCGTAgccgc > 1:1261620/1‑39 (MQ=255) ccTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCtttt > 1:527409/1‑71 (MQ=255) | TTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACTCGATAGCTGGCGTAGCCGCCTGGCGGTAGTTAGCCAGACGCCATTCCTTTT > minE/298474‑298604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |