| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,467,238 | G→T | 16.6% | L234L (CTG→CTT) | atpA → | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,467,238 | 0 | G | T | 16.6% | 98.3 / 5.3 | 36 | L234L (CTG→CTT) | atpA | F1 sector of membrane‑bound ATP synthase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/7); new base T (3/3); total (26/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGA > minE/2467172‑2467308 | ggAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCaa > 1:1270926/1‑70 (MQ=255) gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTTCACTTCAAt < 1:1761504/70‑1 (MQ=255) gAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATa < 1:518883/71‑1 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCg > 1:1111233/1‑71 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg > 1:1982997/1‑71 (MQ=255) gcgcACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCg < 1:37255/71‑1 (MQ=255) cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:169852/1‑71 (MQ=255) cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1167503/1‑71 (MQ=255) cacCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcg > 1:1758674/1‑71 (MQ=255) acCATCGTTGTGGTAGCAACCGCGTCTTAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:2142054/71‑1 (MQ=255) ccATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCa < 1:1820151/71‑1 (MQ=255) ccATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTgcgc < 1:1329941/70‑1 (MQ=255) aTCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAAt > 1:193760/1‑71 (MQ=255) aTCGTTGTGGTAGCAACCGCGTCAGAATCCGCTGCACTGCAATAc > 1:1113052/1‑45 (MQ=255) tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTg > 1:1242036/1‑64 (MQ=255) tCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCCTATGCCGGTTGCGCAAtt > 1:1790039/1‑70 (MQ=255) cGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATgg > 1:1010536/1‑71 (MQ=255) gTTGTGGTAGCAACCGCGTCTGAATCCTCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATggg > 1:992936/1‑71 (MQ=255) ttGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTa > 1:390201/1‑52 (MQ=255) gtgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCg > 1:1702667/1‑70 (MQ=255) tgGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGaa < 1:13102/71‑1 (MQ=255) gTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTg > 1:1079173/1‑39 (MQ=255) aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGtt > 1:297523/1‑50 (MQ=255) aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTcc > 1:1350908/1‑71 (MQ=255) aaCCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTcc < 1:1875194/71‑1 (MQ=255) ccGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCTGTTGCGCAATGGGCGAATACTTCCGt < 1:696996/71‑1 (MQ=255) ccGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt < 1:1969641/71‑1 (MQ=255) gcgTCTGAATCCGCTGCACTTCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:810450/1‑71 (MQ=255) gcgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGa > 1:64501/1‑71 (MQ=255) cgTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGAc > 1:1699021/1‑71 (MQ=255) tCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcg > 1:699777/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1652749/1‑71 (MQ=255) tGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:1707727/1‑71 (MQ=255) gAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACcgcg > 1:2201594/1‑70 (MQ=255) aaTCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGt > 1:1727806/1‑71 (MQ=255) gCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGt > 1:14291/1‑57 (MQ=255) ctgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGAt < 1:1654620/71‑1 (MQ=255) tgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGaa < 1:170733/67‑1 (MQ=255) tgcactgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGaa < 1:1243379/67‑1 (MQ=255) actgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATgcgc > 1:159225/1‑71 (MQ=255) ctgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCt > 1:1777966/1‑71 (MQ=255) tgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:164052/64‑1 (MQ=255) tgcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGa < 1:121828/64‑1 (MQ=255) gcaATACCTGGCACCGTATGCCGTTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:2151513/1‑71 (MQ=255) gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:150039/1‑71 (MQ=255) gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:1400615/1‑71 (MQ=255) gcaATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGa > 1:1044182/1‑71 (MQ=255) | GGAAGAGCACGGCGCACTGGCTAACACCATCGTTGTGGTAGCAACCGCGTCTGAATCCGCTGCACTGCAATACCTGGCACCGTATGCCGGTTGCGCAATGGGCGAATACTTCCGTGACCGCGGTGAAGATGCGCTGA > minE/2467172‑2467308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |