| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,473,718 | C→T | 25.9% | F331F (TTC→TTT) | glmS → | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,473,718 | 0 | C | T | 25.9% | 97.8 / 34.2 | 54 | F331F (TTC→TTT) | glmS | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base T (8/6); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCGCG > minE/2473653‑2473787 | gTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTa > 1:265157/1‑71 (MQ=255)gTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTa < 1:1821348/71‑1 (MQ=255) tCCCGCTAATGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCg > 1:634918/1‑71 (MQ=255) ccGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGc < 1:170253/70‑1 (MQ=255) ccGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGc > 1:1376055/1‑70 (MQ=255) cGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaa < 1:1958927/71‑1 (MQ=255) cTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTcc < 1:1298440/56‑1 (MQ=255) ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaaa < 1:849720/65‑1 (MQ=255) ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGcc > 1:1695648/1‑71 (MQ=255) gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCg > 1:221005/1‑71 (MQ=255) gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTAt > 1:2120803/1‑58 (MQ=255) ttGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTg > 1:248361/1‑71 (MQ=255) tGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTg < 1:1663180/70‑1 (MQ=255) aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCaaa < 1:1890233/59‑1 (MQ=255) aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtc > 1:618196/1‑71 (MQ=255) cGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcg > 1:1042841/1‑70 (MQ=255) cGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcg > 1:145761/1‑70 (MQ=255) gCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTa < 1:1288039/71‑1 (MQ=255) gCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGcgtcgt < 1:316043/70‑1 (MQ=255) gCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACa < 1:1789742/70‑1 (MQ=255) ggTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt < 1:1344888/71‑1 (MQ=255) gTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGc < 1:173402/38‑1 (MQ=255) tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGc > 1:425150/1‑56 (MQ=255) ttCCGTGCCACGTCGAAATCGCCTCTGAATTCCGCTATCGCaa < 1:1514631/43‑1 (MQ=255) ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTa > 1:197205/1‑35 (MQ=255) ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAAt > 1:1836365/1‑43 (MQ=255) ccGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgatga > 1:512219/1‑70 (MQ=255) gCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCa > 1:905600/1‑69 (MQ=255) gACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat > 1:866475/1‑65 (MQ=255) tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgat < 1:2104701/58‑1 (MQ=255) tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgat < 1:775696/58‑1 (MQ=255) gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTcaca < 1:800716/71‑1 (MQ=255) gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:531391/1‑69 (MQ=255) gAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:980424/1‑69 (MQ=255) cGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat > 1:32043/1‑54 (MQ=255) cGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTg > 1:1197213/1‑71 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:234071/61‑1 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:2069471/61‑1 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:1501136/61‑1 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:1867122/61‑1 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGt < 1:777865/61‑1 (MQ=255) gCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTg < 1:455781/70‑1 (MQ=255) ctctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:1875116/1‑61 (MQ=255) ctctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:778080/1‑61 (MQ=255) ctctGAATTCCGCTATAGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca > 1:552105/1‑61 (MQ=255) ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:599468/1‑70 (MQ=255) ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:65789/1‑70 (MQ=255) ctGAATTTCGCTATCGCAAATCAGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg > 1:770892/1‑69 (MQ=255) ctGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa > 1:2039083/1‑70 (MQ=255) gAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:972656/1‑71 (MQ=255) gAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:1673159/1‑71 (MQ=255) gAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAc > 1:1661473/1‑71 (MQ=255) aaTTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTc < 1:1645429/55‑1 (MQ=255) aTTTCGCTATCGCAAATCTGCCTTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAAcc < 1:232499/70‑1 (MQ=255) ttCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTcac > 1:764557/1‑55 (MQ=255) ccGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcgcg < 1:697514/70‑1 (MQ=255) ccGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcgcg < 1:336040/70‑1 (MQ=255) | GTTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCGCG > minE/2473653‑2473787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |