| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,022 | G→A | 8.9% | A281V (GCG→GTG) | mdtL ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,022 | 0 | G | A | 8.9% | 129.2 / 3.9 | 45 | A281V (GCG→GTG) | mdtL | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (19/22); new base A (2/2); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
ACCGGCGCAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATG > minE/2481952‑2482088 | aCCGGCGCAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCccg > 1:128614/1‑71 (MQ=255) ccGGCGCAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCccgc > 1:54908/1‑71 (MQ=255) gcgcAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCcgccgc > 1:1691522/1‑71 (MQ=255) cgcAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCgccgcc < 1:1490279/71‑1 (MQ=255) aaTCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAgg < 1:1088288/70‑1 (MQ=255) aaTCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAgg > 1:1171600/1‑70 (MQ=255) tCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAAt < 1:1851434/71‑1 (MQ=255) aGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGa > 1:193104/1‑67 (MQ=255) aGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAAt < 1:2054980/69‑1 (MQ=255) gCGTGATACCAAACAGAGAAACCGCATGGGAAGGTTAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAac < 1:561633/71‑1 (MQ=255) gTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAacac < 1:994597/71‑1 (MQ=255) gTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAacac < 1:441787/71‑1 (MQ=255) gATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCACCGCCAGGAATaa > 1:1363891/1‑66 (MQ=255) tACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGc < 1:277846/71‑1 (MQ=255) aCCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGaa > 1:1105783/1‑60 (MQ=255) ccAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCg < 1:1197677/70‑1 (MQ=255) ccAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGa < 1:484331/71‑1 (MQ=255) aaaCAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAAt < 1:1388743/58‑1 (MQ=255) aaaCAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAg > 1:243865/1‑70 (MQ=255) aaCAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGt > 1:1177861/1‑71 (MQ=255) agagAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCTAGGTGat < 1:370610/71‑1 (MQ=255) agagAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCACCGCCAGGAATAACACCTGCGAGGTGat < 1:735030/71‑1 (MQ=255) gagaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCACCGCCAGGAATAACACCTGCGAGGTGat < 1:886916/70‑1 (MQ=255) gaAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGatca < 1:1945832/70‑1 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCaa < 1:877213/71‑1 (MQ=255) aCCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCaa < 1:967994/71‑1 (MQ=255) gCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGATTAACACCTGCGAGGTGATCATCAccgt < 1:2006328/71‑1 (MQ=255) aGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCt > 1:2117952/1‑71 (MQ=255) ggTGAAACGGCAAGAGTTATCCCCGCCGCCAGGAAt < 1:1240401/36‑1 (MQ=255) aaaCGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGat < 1:986165/49‑1 (MQ=255) aCGGCAAGAGTGATCCCCACCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAAt > 1:311566/1‑71 (MQ=255) gCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTcc > 1:114525/1‑71 (MQ=255) gCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTcc > 1:893996/1‑71 (MQ=255) gCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTcc > 1:1565349/1‑71 (MQ=255) agagTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAg > 1:2002625/1‑71 (MQ=255) gagTGATCCCCGCCGCCAGTAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgc > 1:386020/1‑71 (MQ=255) agTGATCCCCGCCGCCAGGAATAACACCTGCGAgg > 1:427927/1‑35 (MQ=255) agTGATCCCCGCCGCCAGGAATAACACCTGCGAgg > 1:266233/1‑35 (MQ=255) agTGATCCCCGCCGCCAGGAATAACACCTGCGAgg > 1:804922/1‑35 (MQ=255) agTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgcg < 1:589970/71‑1 (MQ=255) gTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgcg > 1:1378462/1‑70 (MQ=255) gTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAgcg > 1:1924668/1‑70 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa > 1:1213567/1‑71 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa > 1:689687/1‑71 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa < 1:2199174/71‑1 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaaa < 1:1584309/71‑1 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaa < 1:278120/70‑1 (MQ=255) aTCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCaa < 1:241557/70‑1 (MQ=255) ccccGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCATCGCAAATg > 1:1531965/1‑71 (MQ=255) ccgccgCCAGGAATAACACCTCCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCa < 1:1300793/65‑1 (MQ=255) | ACCGGCGCAAATCAGCGTGATACCAAACAGAGAAACCGCATGGGAAGGTGAAACGGCAAGAGTGATCCCCGCCGCCAGGAATAACACCTGCGAGGTGATCATCAACGTACGTGGCTTAAAAATTCCCAGCGCAAATG > minE/2481952‑2482088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |