| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,516,656 | C→T | 31.7% | A102T (GCT→ACT) | trmH ← | tRNA (Guanosine‑2'‑O‑)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,516,656 | 0 | C | T | 31.7% | 116.9 / 68.5 | 82 | A102T (GCT→ACT) | trmH | tRNA (Guanosine‑2'‑O‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/26); new base T (13/13); total (43/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCC > minE/2516587‑2516722 | tGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCg > 1:217726/1‑71 (MQ=255) cccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGtt < 1:351389/71‑1 (MQ=255) cGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTAt > 1:572162/1‑71 (MQ=255) tttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTAt > 1:769719/1‑68 (MQ=255) ttgctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTAAt > 1:947769/4‑68 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAg > 1:224344/1‑70 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAg > 1:315048/1‑70 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAg > 1:818588/1‑70 (MQ=255) ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAg > 1:455176/1‑70 (MQ=255) ctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaa < 1:1441976/70‑1 (MQ=255) ccTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGa > 1:357079/1‑67 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:941523/68‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:681723/68‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAg < 1:59018/69‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAg < 1:1330478/69‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAg < 1:65427/69‑1 (MQ=255) cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAg < 1:1057297/69‑1 (MQ=255) tGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:1446434/67‑1 (MQ=255) ccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGt < 1:247694/70‑1 (MQ=255) cATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGtt < 1:1316309/54‑1 (MQ=255) cATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGaaa < 1:1800844/62‑1 (MQ=255) aTCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:437563/1‑71 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc > 1:819600/1‑69 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGt > 1:784542/1‑50 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGt > 1:781943/1‑50 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGt > 1:125261/1‑50 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:2123479/1‑69 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:1995904/1‑69 (MQ=255) cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGc > 1:2030874/1‑69 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCTACAGCGTTATCAGAAAGATGGGTTGCCAg < 1:1423733/71‑1 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc > 1:2153693/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc > 1:668996/1‑68 (MQ=255) aaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGa < 1:1129427/71‑1 (MQ=255) aaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:329439/71‑1 (MQ=255) aaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGa < 1:731685/70‑1 (MQ=255) aTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGa > 1:2205129/1‑57 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:1484347/71‑1 (MQ=255) cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:1558808/71‑1 (MQ=255) gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:1888660/68‑1 (MQ=255) gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCAt < 1:869091/71‑1 (MQ=255) gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCa < 1:725360/45‑1 (MQ=255) cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCa > 1:712127/1‑68 (MQ=255) cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGc > 1:2150665/1‑71 (MQ=255) ggCGAGTGTAATCAATTTCGCGGAAATCGACAGCTTTATCAGAAAGATggg > 1:404755/1‑51 (MQ=255) ggCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAg < 1:1848019/42‑1 (MQ=255) ggCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATggg > 1:1143610/1‑51 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa < 1:508598/39‑1 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa < 1:407858/39‑1 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt < 1:1553388/57‑1 (MQ=255) tgtAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCt < 1:1342974/57‑1 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:417098/1‑71 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:605846/1‑71 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCtt < 1:1136973/70‑1 (MQ=255) tAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCtt < 1:499035/70‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:1754517/1‑69 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:1188419/1‑69 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga < 1:821362/71‑1 (MQ=255) aTCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga < 1:1898883/71‑1 (MQ=255) tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc < 1:2049904/44‑1 (MQ=255) tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGa < 1:1335671/48‑1 (MQ=255) tCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:2120995/1‑68 (MQ=255) cAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:124272/1‑67 (MQ=255) cAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt > 1:1981479/1‑67 (MQ=255) aaTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCa > 1:1669969/1‑44 (MQ=255) aaTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg < 1:891449/51‑1 (MQ=255) aaTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGAt < 1:638593/71‑1 (MQ=255) aaTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGAt > 1:2164258/1‑71 (MQ=255) aaTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGaa > 1:1367643/1‑47 (MQ=255) aaTTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGaa > 1:2080870/1‑47 (MQ=255) tCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAg > 1:245385/1‑70 (MQ=255) tCGCGGAAATCGACAGCGTTATCAGAAAGATGGGt > 1:468153/1‑35 (MQ=255) tCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGc > 1:985399/1‑71 (MQ=255) tCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCAAGAATCTGCATGCCCTGGCCTTTgaga > 1:782625/1‑66 (MQ=255) ggAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgag < 1:1370269/61‑1 (MQ=255) ggAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgag < 1:1043291/61‑1 (MQ=255) ggAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgag < 1:1030625/61‑1 (MQ=255) aTCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGc < 1:82673/69‑1 (MQ=255) cGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg > 1:244802/1‑71 (MQ=255) agACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAg > 1:758304/2‑60 (MQ=255) gACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc > 1:1039778/1‑71 (MQ=255) aCAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc < 1:1676591/71‑1 (MQ=255) cAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc > 1:751368/1‑70 (MQ=255) | TGCCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCC > minE/2516587‑2516722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |