| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,523,040 | T→C | 31.3% | K225K (AAA→AAG) | dinD ← | DNA‑damage‑inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,523,040 | 0 | T | C | 31.3% | 67.2 / 44.3 | 54 | K225K (AAA→AAG) | dinD | DNA‑damage‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (18/19); new base C (9/7); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
TCCGCCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAGAGATTAGCCG > minE/2522971‑2523104 | tCCGCCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGtt > 1:971193/1‑71 (MQ=255) ccGCCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGttt > 1:1068161/1‑71 (MQ=255) ccGCCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGtt < 1:693157/70‑1 (MQ=255) aaGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGaa < 1:508427/70‑1 (MQ=255) aGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAAtt < 1:994742/71‑1 (MQ=255) ttCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCa < 1:814585/71‑1 (MQ=255) cTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACg < 1:2131351/70‑1 (MQ=255) tGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATcc < 1:127659/71‑1 (MQ=255) gTTTGCCTCACCTTGCGCCCGACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATccc > 1:1153063/1‑70 (MQ=255) gTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCg > 1:101483/1‑71 (MQ=255) gTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGGTTTGAATTCACGCCATccc > 1:763562/1‑70 (MQ=255) tGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCtt > 1:1838577/1‑71 (MQ=255) cACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGc > 1:1083837/1‑56 (MQ=255) aCCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGttt > 1:738492/1‑71 (MQ=255) aCCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGttt > 1:879689/1‑71 (MQ=255) aCCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGttt > 1:1790469/1‑71 (MQ=255) aCCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGtt < 1:1814557/70‑1 (MQ=255) aCCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTAAGttt > 1:2022320/1‑71 (MQ=255) ccTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGtttt < 1:1381657/71‑1 (MQ=255) cTTGCGACCCACGTCAAAGTGTGTGGTTTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGa < 1:550946/65‑1 (MQ=255) cTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTGACGCCATCCCGCTTGAGttttt < 1:1654124/71‑1 (MQ=255) cTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGttttt > 1:57239/1‑71 (MQ=255) cTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGtttt < 1:879971/70‑1 (MQ=255) tGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGaa > 1:1932851/1‑44 (MQ=255) tGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTttct > 1:1843053/1‑71 (MQ=255) tGCGACCCACATCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCa > 1:732297/1‑53 (MQ=255) gCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTttc < 1:1777561/69‑1 (MQ=255) cGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTttcttc < 1:1509159/71‑1 (MQ=255) cACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGttt < 1:2036969/71‑1 (MQ=255) aCGTCAAAGTGTGTGGTGTTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTg > 1:2132259/1‑71 (MQ=255) cGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGttttt > 1:1848512/1‑60 (MQ=255) gTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTggg < 1:809009/71‑1 (MQ=255) tCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTg < 1:313741/64‑1 (MQ=255) tCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTggg < 1:94564/70‑1 (MQ=255) cAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTTAGtt < 1:1058902/54‑1 (MQ=255) tggtgTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGtt > 1:1159180/1‑56 (MQ=255) tggtgTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGtt > 1:987474/1‑56 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTttc > 1:266959/1‑47 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGaaa > 1:2103454/1‑68 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAaga < 1:1141352/70‑1 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAaga < 1:1542925/70‑1 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAaga < 1:1223765/70‑1 (MQ=255) gtgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAaga < 1:1586996/70‑1 (MQ=255) tgtTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTttcttc < 1:1416064/49‑1 (MQ=255) tgtTTGCTTGCTGCTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAgaga < 1:1926416/71‑1 (MQ=255) tttGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGa > 1:584722/1‑37 (MQ=255) tgcttgctGCTTTGAATTCACGCCATCCCGCTTGAGt > 1:1815705/1‑37 (MQ=255) tgcttgctGCTTTGAATTCACGCCATCCCGCTTGAGt > 1:1544772/1‑37 (MQ=255) tgcttgctGCTTTGAATTCACGCCATCCCGCTTGAGt > 1:251723/1‑37 (MQ=255) tgcttgctGCTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAGAGATTAg > 1:882922/1‑71 (MQ=255) cttgctGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAGAGATTAgcc < 1:1427205/71‑1 (MQ=255) ttgctGCTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGa < 1:1703914/58‑1 (MQ=255) tgctGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTggg > 1:263300/1‑49 (MQ=255) tgctGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTggg > 1:1166441/1‑49 (MQ=255) gctgctTTGAATTCACGCCATCCCGCTTGAGTTTTtcttct > 1:1969217/1‑41 (MQ=255) gctgctTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTg < 1:1022612/42‑1 (MQ=255) gctgctTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGaaa > 1:1921320/1‑58 (MQ=255) gctGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAGAGATTAgccg > 1:1824024/1‑69 (MQ=255) ctGCTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTg < 1:24632/41‑1 (MQ=255) tGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAaga > 1:335762/1‑58 (MQ=255) | TCCGCCAAGTTCCTGAATGGTTTGCCTCACCTTGCGACCCACGTCAAAGTGTGTGGTGTTTGCTTGCTGTTTTGAATTCACGCCATCCCGCTTGAGTTTTTCTTCTGTTTGGGTAGCTCGAAAGAGATTAGCCG > minE/2522971‑2523104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |