| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,528,498 | C→T | 21.8% | D83N (GAT→AAT) | dfp ← | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,528,498 | 0 | C | T | 21.8% | 117.1 / 27.0 | 55 | D83N (GAT→AAT) | dfp | fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding |
| Reads supporting (aligned to +/- strand): ref base C (26/17); new base T (7/5); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCG > minE/2528436‑2528562 | cGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAtt > 1:399179/1‑71 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATc < 1:534146/62‑1 (MQ=255) gCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCAttt < 1:1843881/71‑1 (MQ=255) cATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAc > 1:1654053/1‑71 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTa > 1:297880/1‑66 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:1469731/1‑70 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAg > 1:995663/1‑71 (MQ=255) ccGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAg > 1:314257/1‑71 (MQ=255) agcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:1667221/71‑1 (MQ=255) gcaACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTc < 1:1978690/68‑1 (MQ=255) caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:331886/1‑71 (MQ=255) caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:920627/1‑71 (MQ=255) caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:1213465/1‑71 (MQ=255) caACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAat > 1:197468/1‑70 (MQ=255) acacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATg > 1:187874/1‑71 (MQ=255) cacGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCAttt < 1:1184573/55‑1 (MQ=255) acGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCACATTTACCCAGCTCAata > 1:2194052/1‑67 (MQ=255) cGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAATATGGcc < 1:585091/71‑1 (MQ=255) aTCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTa < 1:558972/48‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:70315/57‑1 (MQ=255) tCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCa < 1:61884/43‑1 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCa > 1:957722/1‑50 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCa > 1:1903493/1‑65 (MQ=255) cAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCg > 1:909106/1‑70 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:509682/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATTAGCCCATTTACCCAGCTCAata > 1:2214075/1‑57 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATTGcc > 1:1899626/1‑62 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:616752/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1104866/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:2199288/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1056121/1‑71 (MQ=255) aaaTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGc > 1:1597189/1‑71 (MQ=255) aTCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCtt < 1:2085266/71‑1 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGc > 1:1411687/1‑35 (MQ=255) tCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGc > 1:847287/1‑35 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCa < 1:1626797/49‑1 (MQ=255) tGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCa < 1:1593899/49‑1 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTAccc > 1:689082/1‑41 (MQ=255) ccGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:428948/71‑1 (MQ=255) gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCaa < 1:732650/46‑1 (MQ=255) gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGc < 1:1776820/71‑1 (MQ=255) gTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGc < 1:1187164/71‑1 (MQ=255) tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:1875935/68‑1 (MQ=255) tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCt < 1:1873274/68‑1 (MQ=255) tGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGcc > 1:114650/1‑71 (MQ=255) cAGGGGCGAGAATCACTAAATCAGCCCATTTACACAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCggg < 1:671515/71‑1 (MQ=255) ggggCGAGAATCACTAAATTAGCCCATTTACCCAGCTCaa < 1:1652953/40‑1 (MQ=255) gggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTc > 1:1393538/1‑70 (MQ=255) ggCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCca < 1:2125876/71‑1 (MQ=255) gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:162344/1‑39 (MQ=255) gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAata > 1:507750/1‑39 (MQ=255) gCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcag < 1:1601090/71‑1 (MQ=255) gagaATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCcagca < 1:555005/71‑1 (MQ=255) aCTAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTc > 1:260737/1‑71 (MQ=255) tAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCg > 1:277525/1‑70 (MQ=255) tAAATTAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCg > 1:2103672/1‑70 (MQ=255) | CGCCATTCCGGCAGCAACACGGGCAATCAAATCTGCCGTGGCAGGGGCGAGAATCACTAAATCAGCCCATTTACCCAGCTCAATATGGCCCATAGCGGCTTCGGCTGCCGGGTCCAGCAGACTGTCG > minE/2528436‑2528562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |