| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,531,609 | A→G | 7.3% | I14I (ATT→ATC) | coaD ← | pantetheine‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,531,609 | 0 | A | G | 7.3% | 140.2 / 4.2 | 55 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (27/24); new base G (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
CGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAG > minE/2531546‑2531678 | cGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGAt > 1:610997/1‑71 (MQ=255) gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1157188/1‑71 (MQ=255) cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACAATTGGTGATGGGATCGa < 1:205095/71‑1 (MQ=255) gTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg > 1:603254/1‑71 (MQ=255) gAACATCTGGGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1742911/56‑1 (MQ=255) gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:198460/56‑1 (MQ=255) aaCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATa < 1:1178738/68‑1 (MQ=255) cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaaa > 1:1978680/1‑68 (MQ=255) tCTGGGTGGCGCGCGTCACGATATCGATATGACCATTGGTaa < 1:252961/42‑1 (MQ=255) tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1666656/51‑1 (MQ=255) tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:160429/1‑71 (MQ=255) cGTGTCGCGAGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg < 1:2011464/69‑1 (MQ=255) gTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttt < 1:1036816/71‑1 (MQ=255) tGGCGCGCGTCACGATATCGATATGACCATTGGTa < 1:1527863/35‑1 (MQ=255) tGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:137778/45‑1 (MQ=255) tGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:1456016/71‑1 (MQ=255) gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg > 1:2073313/1‑71 (MQ=255) gcgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:2107791/1‑57 (MQ=255) cgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt < 1:1365663/71‑1 (MQ=255) gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc > 1:524569/1‑46 (MQ=255) gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc > 1:994376/1‑46 (MQ=255) cgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa < 1:1844277/70‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg < 1:397179/45‑1 (MQ=255) tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca < 1:1823148/71‑1 (MQ=255) cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttttt > 1:1091958/1‑62 (MQ=255) cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:572944/1‑66 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:1920187/1‑71 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac < 1:926144/71‑1 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac < 1:871002/71‑1 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:521012/1‑71 (MQ=255) aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaa > 1:1011184/1‑67 (MQ=255) gatatcgatatGACCATTGGTAATGGGATCGAAAg > 1:1659635/1‑35 (MQ=255) atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGAt > 1:624736/1‑43 (MQ=255) tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:5634/64‑1 (MQ=255) tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc > 1:768265/1‑70 (MQ=255) tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa < 1:2126304/71‑1 (MQ=255) tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa < 1:1213639/71‑1 (MQ=255) tatagatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt > 1:1756603/1‑69 (MQ=255) atcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:2096652/1‑71 (MQ=255) gatatGAGCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1261719/50‑1 (MQ=255) gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa > 1:975820/1‑67 (MQ=255) gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc < 1:954322/71‑1 (MQ=255) atGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGtt > 1:839521/1‑71 (MQ=255) tGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:62130/36‑1 (MQ=255) aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca > 1:475426/1‑55 (MQ=255) aTTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa < 1:903687/49‑1 (MQ=255) ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:435397/67‑1 (MQ=255) tGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:666936/1‑51 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:465735/1‑50 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:268945/1‑50 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:1244307/1‑50 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:2184536/1‑50 (MQ=255) ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAAt > 1:356305/1‑57 (MQ=255) ggTAATGGGATCGAAAGTACCCCGATAAATCGCCCGTTTTTGCATAACAACCTCAAt > 1:2062609/1‑57 (MQ=255) tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGttt < 1:1488684/61‑1 (MQ=255) tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1285584/63‑1 (MQ=255) tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAg < 1:157506/71‑1 (MQ=255) aaTGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:694589/1‑53 (MQ=255) | CGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAG > minE/2531546‑2531678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |