| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,540,373 | G→T | 8.1% | D300Y (GAT→TAT) | rfaJ → | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,540,373 | 0 | G | T | 8.1% | 223.1 / 3.2 | 73 | D300Y (GAT→TAT) | rfaJ | UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (32/35); new base T (2/4); total (34/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
AATTTATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAT > minE/2540303‑2540437 | aaTTTATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACgag < 1:1542309/71‑1 (MQ=255) tttATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGAt > 1:1743338/1‑71 (MQ=255) tATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc > 1:1793322/1‑71 (MQ=255) aTCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGc > 1:2180638/1‑70 (MQ=255) tCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATc < 1:1200932/71‑1 (MQ=255) cGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATc > 1:2046395/1‑70 (MQ=255) ggTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCaa > 1:1650913/1‑71 (MQ=255) gTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATCAat < 1:1065224/71‑1 (MQ=255) tAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACgaga > 1:1644190/1‑58 (MQ=255) aTACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattatt > 1:1754486/1‑70 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAttt > 1:490746/1‑70 (MQ=255) taAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAAttt > 1:254619/1‑70 (MQ=255) aaaTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGGATTTaaa < 1:1865812/71‑1 (MQ=255) aTAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaa < 1:1535846/71‑1 (MQ=255) tAGCTTTAGAAAATTCCACCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaaa < 1:1716269/71‑1 (MQ=255) aGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaa > 1:1220931/1‑69 (MQ=255) aGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaa > 1:1126126/1‑69 (MQ=255) gCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaaaa > 1:1768042/1‑68 (MQ=255) tAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGCTGCGAAATCAat > 1:2101095/1‑49 (MQ=255) aGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGatat < 1:981856/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGATATGCGAAATc < 1:1599662/44‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1224608/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1692057/70‑1 (MQ=255) gAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:622345/70‑1 (MQ=255) aaaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaaa < 1:865767/71‑1 (MQ=255) aaaTTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCa < 1:898284/43‑1 (MQ=255) aTTCCCCCTGGAAAGATGACTCTCCACGAGATGCTAAATCAATTATTGAATTTAAAAAAAGATATAAACAt < 1:1887837/71‑1 (MQ=255) ttCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATc > 1:907496/1‑71 (MQ=255) ttCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATc < 1:2003367/71‑1 (MQ=255) tCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattatt > 1:96771/1‑45 (MQ=255) tCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattatt > 1:2031120/1‑45 (MQ=255) tCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAattatt > 1:853110/1‑45 (MQ=255) tCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTa < 1:2136476/52‑1 (MQ=255) cccccTGGAAAGATCACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCt < 1:1874781/70‑1 (MQ=255) ccccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:326074/71‑1 (MQ=255) cccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTaaa < 1:1745742/51‑1 (MQ=255) cccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:565148/71‑1 (MQ=255) cccTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCtttt < 1:2080703/71‑1 (MQ=255) tGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:1280267/35‑1 (MQ=255) tGGAAAGATGACTCTCCACGAGATGCGAAATCAat < 1:801088/35‑1 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGaa > 1:18407/1‑41 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt > 1:1361532/1‑66 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:1259845/1‑68 (MQ=255) ggAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg > 1:960633/1‑71 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTg < 1:1866024/70‑1 (MQ=255) gAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:746978/71‑1 (MQ=255) aaGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGAtata < 1:1253262/54‑1 (MQ=255) aaGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCaa > 1:1133901/1‑71 (MQ=255) aGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAg < 1:2061715/48‑1 (MQ=255) tGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGa < 1:953186/46‑1 (MQ=255) gACTCTCCACGATATGCGTAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTa > 1:474323/1‑60 (MQ=255) gACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGa > 1:1367700/1‑45 (MQ=255) gACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:719820/58‑1 (MQ=255) gACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:1348476/58‑1 (MQ=255) tctcCACTAGATGCGAAATCAATTATTGAATTTaaaaaa < 1:1020790/39‑1 (MQ=255) tctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaa < 1:1407884/47‑1 (MQ=255) tctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaa < 1:650983/47‑1 (MQ=255) tctcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATta > 1:1760113/1‑71 (MQ=255) tcCACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGc < 1:792237/59‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:318203/53‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCttt < 1:1861943/53‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCt < 1:558724/51‑1 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAg > 1:1153897/1‑56 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAg > 1:1447852/1‑56 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAg > 1:1747477/1‑56 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:1797951/1‑71 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:1406636/1‑71 (MQ=255) tcCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtata > 1:1521290/1‑71 (MQ=255) ccACGATATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTatat > 1:1568589/1‑71 (MQ=255) ccACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaa < 1:2181059/44‑1 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata > 1:1981406/1‑40 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaaa > 1:1333483/1‑44 (MQ=255) cACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATtat < 1:2162037/68‑1 (MQ=255) aCGAGATGCGAAATCAATTATTGAATTTAAAAAAAGata < 1:1227449/39‑1 (MQ=255) cGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATaaa > 1:217619/1‑42 (MQ=255) | AATTTATCCATCGGTAAAATACTATAAAATAGCTTTAGAAAATTCCCCCTGGAAAGATGACTCTCCACGAGATGCGAAATCAATTATTGAATTTAAAAAAAGATATAAACATCTTTTAGTGCAACATCATTATAT > minE/2540303‑2540437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |