| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 300,220 | G→A | 25.0% | L372L (CTG→CTA) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 300,220 | 0 | G | A | 25.0% | 73.2 / 22.8 | 40 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (6/4); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGG > minE/300150‑300287 | cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:379275/71‑1 (MQ=255) tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCg < 1:1536678/71‑1 (MQ=255) cgcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCgg > 1:1343188/1‑71 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1082135/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg > 1:268813/1‑71 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1224123/71‑1 (MQ=255) gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:631516/71‑1 (MQ=255) gACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:990887/66‑1 (MQ=255) cAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCg > 1:1319079/1‑71 (MQ=255) gCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1258108/55‑1 (MQ=255) gCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGc > 1:1168154/1‑70 (MQ=255) cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCgg < 1:618410/53‑1 (MQ=255) cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCa > 1:944209/1‑70 (MQ=255) aaaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:19515/1‑69 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg < 1:2137823/71‑1 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg < 1:1255028/71‑1 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCa > 1:1116827/1‑53 (MQ=255) aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaa < 1:2163275/69‑1 (MQ=255) aaCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa > 1:1087537/1‑67 (MQ=255) aCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc > 1:950404/1‑71 (MQ=255) aTTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGt < 1:141211/41‑1 (MQ=255) tAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCg > 1:1445784/1‑71 (MQ=255) aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAg > 1:1088819/1‑53 (MQ=255) aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc > 1:1382028/1‑71 (MQ=255) tctctcTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc < 1:156239/64‑1 (MQ=255) ctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:1332957/1‑71 (MQ=255) ctctGCGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAc > 1:1915641/1‑60 (MQ=255) tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCt > 1:263780/1‑62 (MQ=255) tgtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:1583097/68‑1 (MQ=255) tgtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:567435/68‑1 (MQ=255) tgtgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAt > 1:1078274/1‑71 (MQ=255) gtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:979638/67‑1 (MQ=255) tgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAtt > 1:1300302/1‑70 (MQ=255) tgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTGtt < 1:887244/70‑1 (MQ=255) gCCTTCGAGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1858986/1‑71 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:345946/71‑1 (MQ=255) ccTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:287821/71‑1 (MQ=255) cgcgCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc > 1:1368858/1‑71 (MQ=255) cAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAtt < 1:1234831/71‑1 (MQ=255) ttttGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAtt < 1:737735/57‑1 (MQ=255) gcTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACgg < 1:622717/71‑1 (MQ=255) | CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGG > minE/300150‑300287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |