| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,605,272 | A→G | 31.5% | L50L (CTT→CTC) | uspA ← | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,605,272 | 0 | A | G | 31.5% | 65.5 / 47.6 | 54 | L50L (CTT→CTC) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base A (19/18); new base G (3/14); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAG > minE/2605209‑2605335 | ggTCAGTGCATGATGTTTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCg < 1:1154830/70‑1 (MQ=255)ggTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCgg > 1:2153935/1‑71 (MQ=255) tCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGgtg < 1:750460/71‑1 (MQ=255) tGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGtata < 1:1151697/71‑1 (MQ=255) tGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGtata < 1:356908/71‑1 (MQ=255) atgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGt > 1:1516326/1‑68 (MQ=255) tttgtCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt < 1:1495131/59‑1 (MQ=255) tgtgTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt < 1:2205481/61‑1 (MQ=255) tgtgTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt < 1:547031/61‑1 (MQ=255) tgtCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGagt < 1:1474070/71‑1 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGccc < 1:1449643/52‑1 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCa < 1:2190916/65‑1 (MQ=255) ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtg < 1:310152/52‑1 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGttt < 1:721132/68‑1 (MQ=255) agaTGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCt < 1:1842206/71‑1 (MQ=255) gCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGtata > 1:443634/1‑47 (MQ=255) ttCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGagta < 1:1041374/53‑1 (MQ=255) ttCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:862336/1‑71 (MQ=255) tCTGCATATCACCCAGATTCACATAAATAAGCCCGGTGTATAGGTCAGAGTAGTTTAc > 1:1893782/1‑58 (MQ=255) cATATCACCCAGATTCACATGAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa > 1:2016927/1‑53 (MQ=255) cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa > 1:1954542/1‑53 (MQ=255) atatCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCagag > 1:316307/1‑70 (MQ=255) atatCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAgaga < 1:608444/71‑1 (MQ=255) tatCACCCAGATTCACATCAATAAGCCCGGTGtat < 1:131857/35‑1 (MQ=255) atCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:1882699/1‑63 (MQ=255) tCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGTATCAGAGAAAc > 1:2157450/1‑71 (MQ=255) tCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa > 1:782918/1‑64 (MQ=255) tCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa > 1:324915/1‑64 (MQ=255) tCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc < 1:1459579/71‑1 (MQ=255) tCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc > 1:79777/1‑71 (MQ=255) cACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATc > 1:1014366/1‑52 (MQ=255) cACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATc > 1:1283663/1‑52 (MQ=255) cACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt > 1:696500/1‑71 (MQ=255) cACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt > 1:141140/1‑71 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1410160/70‑1 (MQ=255) aCCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACtt < 1:544154/71‑1 (MQ=255) aGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:2175968/1‑56 (MQ=255) gATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGt < 1:108419/51‑1 (MQ=255) aTTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGt < 1:1017184/50‑1 (MQ=255) aTTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAt < 1:1572346/71‑1 (MQ=255) aTTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAt > 1:1463623/1‑71 (MQ=255) aTTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAt > 1:1461022/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGtt < 1:595565/38‑1 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGtt < 1:1261680/38‑1 (MQ=255) ttCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACa > 1:971626/1‑42 (MQ=255) ttCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:1296604/1‑71 (MQ=255) cacaTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTg < 1:130532/70‑1 (MQ=255) acaTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTa < 1:135305/71‑1 (MQ=255) acaTCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCTCATTGt < 1:644819/70‑1 (MQ=255) acaTCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTg < 1:2096503/69‑1 (MQ=255) caTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAg < 1:2154392/71‑1 (MQ=255) caTCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACtt < 1:1465092/60‑1 (MQ=255) caTCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:1288417/64‑1 (MQ=255) caTCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCa < 1:1814009/65‑1 (MQ=255) | GGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAG > minE/2605209‑2605335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |