| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,632,970 | A→G | 55.6% | K421R (AAA→AGA) | livM → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,632,970 | 0 | A | G | 55.6% | 8.1 / 31.4 | 27 | K421R (AAA→AGA) | livM | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/7); new base G (6/9); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCCTGCTG > minE/2632900‑2633039 | atgatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGaa < 1:1609891/71‑1 (MQ=255) cTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag > 1:2020022/1‑68 (MQ=255) gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCgagaggagag > 1:1760883/1‑70 (MQ=255) gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAgg < 1:1793727/66‑1 (MQ=255) tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAgg > 1:221127/1‑71 (MQ=255) tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAgg < 1:2051450/71‑1 (MQ=255) tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAgg < 1:1683645/71‑1 (MQ=255) cGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCa > 1:411041/1‑71 (MQ=255) cGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCa < 1:1309367/71‑1 (MQ=255) gctgcCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGc > 1:2131236/1‑70 (MQ=255) ccATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGACCAGGCATGAGTCAGCCattat < 1:1005140/71‑1 (MQ=255) cATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCattat > 1:1594243/1‑70 (MQ=255) cgcgCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCa > 1:1194476/1‑46 (MQ=255) gcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt < 1:674347/70‑1 (MQ=255) gcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt < 1:1103875/70‑1 (MQ=255) ccGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt < 1:1340427/65‑1 (MQ=255) ccGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt < 1:1652878/65‑1 (MQ=255) ccGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt < 1:1740768/65‑1 (MQ=255) ccGCAACTGAAGCTGAAAAA‑GGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt < 1:1350621/64‑1 (MQ=255) cGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCat < 1:542865/56‑1 (MQ=255) cGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCat < 1:2148107/56‑1 (MQ=255) tcAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCattatt < 1:2025757/58‑1 (MQ=255) cTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt > 1:1127922/1‑62 (MQ=255) cTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGt > 1:2090310/1‑61 (MQ=255) cTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtg < 1:1295932/71‑1 (MQ=255) gAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTaa > 1:2175123/1‑62 (MQ=255) gAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCtgat > 1:667225/1‑71 (MQ=255) aaaCGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCtt > 1:688408/1‑71 (MQ=255) ggCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCt < 1:2080212/44‑1 (MQ=255) aGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACgg > 1:1031180/1‑47 (MQ=255) aaaGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCctgctg > 1:144120/1‑71 (MQ=255) aaaGGAGAGCAGGCATGAGTAAGCCATTATTATCTGtt < 1:1069541/38‑1 (MQ=255) | ATGATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGCCTGATGATGCGCTTCGGCGGCCTGCTG > minE/2632900‑2633039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |