| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,639,808 | A→G | 20.6% | T245A (ACG→GCG) *147Q (TAA→CAA) |
ugpQ → yhhA ← |
glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,639,808 | 0 | A | G | 20.6% | 69.1 / 13.8 | 34 | T245A (ACG→GCG) *147Q (TAA→CAA) | ugpQ yhhA | glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (13/14); new base G (4/3); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGTTT > minE/2639749‑2639875 | tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAg > 1:1090623/1‑71 (MQ=255)tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAg > 1:1088029/1‑71 (MQ=255) tGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGtttt > 1:657053/1‑70 (MQ=255) ggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCaa < 1:1488044/70‑1 (MQ=255) gtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACgg > 1:1992948/1‑70 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATg > 1:261462/1‑71 (MQ=255) ttGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAAt < 1:1578955/53‑1 (MQ=255) ttGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGt > 1:977585/1‑70 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa < 1:391165/70‑1 (MQ=255) aTCTGTACCGATGCGATTGACGTCATTGGTCCGAACTTTACGGCCCAAt < 1:516858/49‑1 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTg > 1:350756/1‑71 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGaa > 1:2114321/1‑60 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTgc > 1:969612/1‑71 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTgc > 1:543419/1‑71 (MQ=255) tACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGa < 1:249334/57‑1 (MQ=255) ccGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTgcggcg > 1:8798/1‑71 (MQ=255) ccGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTG‑GGCGt > 1:546245/1‑71 (MQ=255) tGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt < 1:750343/71‑1 (MQ=255) gCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTg > 1:868042/1‑71 (MQ=255) cGATTGACGTGATTGGTCCGAACTTTACGGCCCaa < 1:2023457/35‑1 (MQ=255) tGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:774734/1‑70 (MQ=255) aCGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:239222/70‑1 (MQ=255) aCGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa < 1:1342165/70‑1 (MQ=255) cGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTg < 1:174307/60‑1 (MQ=255) cGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTg < 1:843575/60‑1 (MQ=255) gTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGAtt > 1:2019840/1‑70 (MQ=255) tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt < 1:81626/58‑1 (MQ=255) tCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATg < 1:517849/69‑1 (MQ=255) cGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGc > 1:2123621/1‑71 (MQ=255) gAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGt < 1:832586/47‑1 (MQ=255) aaCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg < 1:2020119/36‑1 (MQ=255) aCTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGc > 1:1601643/1‑68 (MQ=255) tttGCGGCGCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAg < 1:1205423/58‑1 (MQ=255) tACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGttt < 1:1399203/69‑1 (MQ=255) | TGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGTTT > minE/2639749‑2639875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |