| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,651,936 | C→T | 15.1% | A57V (GCG→GTG) | glgC → | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,651,936 | 0 | C | T | 15.1% | 140.7 / 14.3 | 53 | A57V (GCG→GTG) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/15); new base T (3/5); total (33/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTC > minE/2651866‑2652004 | aaGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgc < 1:22006/71‑1 (MQ=255) aGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgcg > 1:971541/1‑71 (MQ=255) tAACCAATAAGCGAGCAAAACCAGCCGTACACTTTGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTc > 1:1271472/1‑70 (MQ=255) aaCCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCt > 1:87949/1‑70 (MQ=255) cAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgcg < 1:442735/61‑1 (MQ=255) cAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgcg < 1:965176/61‑1 (MQ=255) cAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACt > 1:241223/1‑71 (MQ=255) cAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACt > 1:618241/1‑71 (MQ=255) aGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCAt > 1:1873987/1‑70 (MQ=255) ccGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTaa > 1:99543/1‑53 (MQ=255) cGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATc < 1:462849/71‑1 (MQ=255) gTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCTCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:1496148/71‑1 (MQ=255) gTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATc < 1:100056/54‑1 (MQ=255) gTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATc < 1:1698225/54‑1 (MQ=255) gTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcg < 1:969690/71‑1 (MQ=255) tACACTTCGGCGGTAAGTTCCGCATTATCGACTTTgc < 1:1496901/37‑1 (MQ=255) acacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCaa > 1:642572/1‑54 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGTGCTGTCTAAc > 1:1917790/1‑45 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACt > 1:342725/1‑46 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACt > 1:1271905/1‑46 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa < 1:1967721/70‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa < 1:1353666/70‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:827145/71‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:1725065/71‑1 (MQ=255) cacTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:1967747/71‑1 (MQ=255) ggcggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGt > 1:555663/1‑71 (MQ=255) gcggTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTg < 1:159919/71‑1 (MQ=255) cggTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATg > 1:565539/1‑64 (MQ=255) cggTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATg > 1:1765545/1‑64 (MQ=255) gTAAGTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTAt < 1:817757/61‑1 (MQ=255) tAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCa > 1:1291389/1‑71 (MQ=255) aaGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGa > 1:347578/1‑67 (MQ=255) aaGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCAc > 1:1865985/1‑71 (MQ=255) aaGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCAc > 1:123722/1‑71 (MQ=255) aGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCAcc < 1:618668/71‑1 (MQ=255) gTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:1684846/55‑1 (MQ=255) gTTCCGCATTATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:445937/55‑1 (MQ=255) gTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtc < 1:2170352/53‑1 (MQ=255) cATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGt < 1:1064715/57‑1 (MQ=255) aTTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcat > 1:666027/1‑46 (MQ=255) aTTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcat > 1:663513/1‑46 (MQ=255) ttATCGACTTTGTGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGt < 1:1567905/66‑1 (MQ=255) tATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTa > 1:1557223/1‑47 (MQ=255) aTCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:1725831/1‑71 (MQ=255) aTCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:664178/1‑71 (MQ=255) aTCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCAACCAGTACCAGTc > 1:1688373/1‑71 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtc > 1:1101894/1‑41 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtc > 1:1497430/1‑41 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCcgtc > 1:1921538/1‑41 (MQ=255) cGACTTTGCGCTGTCTAACTGCATCAACTCAGGGATCcgtc > 1:1844160/1‑41 (MQ=255) aCTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:1891387/1‑67 (MQ=255) aCTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:156671/1‑67 (MQ=255) aCTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTc > 1:1893915/1‑67 (MQ=255) tttGCGCTGTCTAACTGCATCAACTCCGGGATCcgtcgt < 1:1862464/39‑1 (MQ=255) tGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCa > 1:779260/1‑50 (MQ=255) tGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCa > 1:1640009/1‑50 (MQ=255) tGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCacac < 1:389411/69‑1 (MQ=255) tGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACActc > 1:855974/1‑71 (MQ=255) | AAGGATTTAACCAATAAGCGAGCAAAACCGGCCGTACACTTCGGCGGTAAGTTCCGCATTATCGACTTTGCGCTGTCTAACTGCATCAACTCCGGGATCCGTCGTATGGGCGTGATCACCCAGTACCAGTCCCACACTC > minE/2651866‑2652004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |