| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,681,918 | A→G | 25.0% | R598R (CGT→CGC) | yhgF ← | predicted transcriptional accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,681,918 | 0 | A | G | 25.0% | 119.1 / 45.2 | 68 | R598R (CGT→CGC) | yhgF | predicted transcriptional accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (31/20); new base G (10/7); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CTTTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGTT > minE/2681851‑2681982 | ctTTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACg > 1:948753/1‑70 (MQ=255)ctTTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACg > 1:2076590/1‑70 (MQ=255) tgatgaTGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCa < 1:636126/69‑1 (MQ=255) tgatgaTGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt > 1:93500/1‑71 (MQ=255) tgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCa > 1:2189147/1‑63 (MQ=255) ggTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGt > 1:77709/1‑71 (MQ=255) aCCGTCTGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTAcc > 1:2160855/1‑71 (MQ=255) aCCGTCGGCACAGCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTAcc > 1:790541/1‑71 (MQ=255) aCCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTAcc > 1:2070284/1‑71 (MQ=255) aCCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTAcc > 1:497712/1‑71 (MQ=255) gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt > 1:682563/1‑67 (MQ=255) gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAg < 1:1924205/70‑1 (MQ=255) gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGa < 1:6214/71‑1 (MQ=255) gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCAt < 1:719296/67‑1 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAg < 1:1082963/47‑1 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCa > 1:1520501/1‑46 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCa > 1:1666224/1‑46 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCa > 1:1881391/1‑46 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATc > 1:421416/1‑71 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATc > 1:995069/1‑71 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATc > 1:819134/1‑71 (MQ=255) acacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATc > 1:1510788/1‑71 (MQ=255) cacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTAcc < 1:361095/61‑1 (MQ=255) cacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTAcc < 1:734022/61‑1 (MQ=255) cacCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCt < 1:1064387/71‑1 (MQ=255) acCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCgct < 1:1046910/51‑1 (MQ=255) acCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCgct < 1:1804547/51‑1 (MQ=255) acCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCgct < 1:1090134/51‑1 (MQ=255) acCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCgct < 1:1366975/51‑1 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt > 1:1349892/1‑71 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:2142827/71‑1 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:1402195/71‑1 (MQ=255) gAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgctgct > 1:247493/1‑51 (MQ=255) aaTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTAc < 1:2202514/37‑1 (MQ=255) aaTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTAc < 1:133103/37‑1 (MQ=255) aaTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTAc < 1:183541/37‑1 (MQ=255) tctcATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTc > 1:1616467/1‑65 (MQ=255) atcaGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCttt > 1:725343/1‑60 (MQ=255) tcaGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgctg > 1:1879055/1‑71 (MQ=255) caGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgc < 1:249669/36‑1 (MQ=255) aGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGAGCCTGCtgtg > 1:17788/1‑71 (MQ=255) tAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATc < 1:679593/52‑1 (MQ=255) aaaGTCAGACGCTTTCAGGTTACGCAGTTCgctga > 1:1977767/1‑34 (MQ=255) aaaGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGt > 1:670909/1‑58 (MQ=255) aaGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCtgtg > 1:2036756/1‑67 (MQ=255) tCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc < 1:1389911/71‑1 (MQ=255) aGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCa > 1:583835/1‑50 (MQ=255) gACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCtgt < 1:265106/60‑1 (MQ=255) gACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCtgt < 1:480038/60‑1 (MQ=255) aCGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAg < 1:2077270/70‑1 (MQ=255) cGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa > 1:2176164/1‑71 (MQ=255) gCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAg > 1:1776155/1‑47 (MQ=255) gCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAg > 1:77567/1‑47 (MQ=255) gCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgc > 1:909213/1‑62 (MQ=255) gCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa > 1:198640/1‑70 (MQ=255) tttCAGGTTACGCAGTTCGCTGCTGTTACCCATCAg > 1:179431/1‑36 (MQ=255) tttCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGa > 1:1594411/1‑37 (MQ=255) tttCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa > 1:1501396/1‑68 (MQ=255) tCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAg > 1:243219/1‑43 (MQ=255) cAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGtt > 1:1957752/1‑71 (MQ=255) cAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGt > 1:1182380/1‑43 (MQ=255) cAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg > 1:1624570/1‑69 (MQ=255) cAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg > 1:2086580/1‑69 (MQ=255) cAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTAAGt > 1:1576239/1‑43 (MQ=255) ggTTACGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:1080742/37‑1 (MQ=255) ggTTACGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:182069/37‑1 (MQ=255) ttACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAg < 1:771837/59‑1 (MQ=255) ttACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAg < 1:155397/59‑1 (MQ=255) | CTTTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGTT > minE/2681851‑2681982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |